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验证数据展示

  • IF Staining of HepG2 using CL488-20634

    IF Staining of HepG2 using CL488-20634

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 CXCR2 antibody (CL488-20634) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CXCR2 Polyclonal antibody

CXCR2 Polyclonal Antibody for IF/ICC
Cat No. CL488-20634

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

CD182, CDw128b, CMKAR2, CXC R2, CXCR 2, CXCR2, GRO/MGSA receptor, IL 8 receptor type 2, IL 8R B, IL8R2, IL8RA, IL8RB, interleukin 8 receptor, beta

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 20634-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-20634 targets CXCR2 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原

CatNo: Ag14670

Product name: Recombinant human IL-8RB/CXCR2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-57 aa of BC037961

Sequence: MEDFNMESDSFEDFWKGEDLSNYSYSSTLPPFLLDAAPCEPESLEINKYFVVIIYAL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 interleukin 8 receptor, beta
别名 CD182, CDw128b, CMKAR2, CXC R2, CXCR 2, CXCR2, GRO/MGSA receptor, IL 8 receptor type 2, IL 8R B, IL8R2, IL8RA, IL8RB, interleukin 8 receptor, beta
计算分子量 360 aa, 41 kDa
观测分子量 45-50 kDa
GenBank蛋白编号BC037961
基因名称 IL8RB
Gene ID (NCBI) 3579
RRIDAB_3084037
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP25025
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IL8RB, also named as CXCR2, GRO/MGSA receptor, IL-8 receptor type 2, CDw128b and CD182, belongs to the G-protein coupled receptor 1 family. It is a receptor for interleukin-8 which is a powerful neutrophil chemotactic factor. Binding of IL-8 to the receptor causes activation of neutrophils. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. It binds to IL-8 with high affinity. IL8RB also binds with high affinity to CXCL3, GRO/MGSA and NAP-2. This antibody is specific to human IL8RB.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CXCR2 antibody CL488-20634Download protocol
Standard Protocols
Click here to view our Standard Protocols
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