验证数据展示
经过测试的应用
| Positive FC (Intra) detected in | Transfected HEK-293T cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
98497-3-RR targets CXCL7/PPBP in FC (Intra) applications and shows reactivity with rat samples.
| 经测试应用 | FC (Intra) Application Description |
| 经测试反应性 | rat |
| 免疫原 |
CatNo: Eg3405 Product name: Recombinant Rat CXCL7/PPBP protein (rFc Tag) Source: mammalian cells-derived, pHZ-KIsec-N-rFc Tag: N-rFc Domain: 46-107 aa of NM_153721.1 Sequence: IELRCRCTNTLSGIPLNSISRVNVFRPGAHCDNVEVIATLKNGKEVCLDPTAPMIKKIVKKI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | pro-platelet basic protein (chemokine (C-X-C motif) ligand 7) |
| 别名 | Chemokine (C-X-C motif) ligand 7, Cxcl7, Ppbp |
| 计算分子量 | 12 kDa |
| GenBank蛋白编号 | NM_153721.1 |
| 基因名称 | Ppbp |
| Gene ID (NCBI) | 246358 |
| RRID | AB_3746127 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q99ME0 |
| 储存缓冲液 | PBS with 0.09% sodium azide, pH 7.3. |
| 储存条件 | Store at 2 - 8°C. Stable for one year after shipment. |
背景介绍
PPBP, slso named as NAP2, or β-Thromboglobulin, is a platelet-derived growth factor that belongs to the CXC chemokine family. This growth factor is a potent chemoattractant and activator of neutrophils. It has been shown to stimulate various cellular processes including DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by synovial cells.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CXCL7/PPBP antibody 98497-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
