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验证数据展示

  • FC experiment of Jurkat using CL488-17402

    FC experiment of Jurkat using CL488-17402

    1X10^6 Jurkat cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human CXCL12/SDF-1 (CL488-17402) (red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated CXCL12/SDF-1 Polyclonal antibody

CXCL12/SDF-1 Polyclonal Antibody for FC (Intra)
Cat No. CL488-17402

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

FC (Intra)

别名

C X C motif chemokine 12, CXCL12, CXCL12/SDF-1, hIRH, hSDF 1, IRH, PBSF, SCYB12, SDF 1, SDF 1a, SDF 1b, SDF1, SDF-1, SDF1A, SDF1B, Stromal cell derived factor 1, TLSF a, TLSF b, TPAR1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inJurkat cells
Positive FC detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 17402-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17402 targets CXCL12/SDF-1 in FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag11379

Product name: Recombinant human CXCL12 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 19-89 aa of BC039893

Sequence: SDGKPVSLSYRCPCRFFESHVARANVKHLKILNTPNCALQIVARLKNNNRQVCIDPKLKWIQEYLEKALNK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1)
别名 C X C motif chemokine 12, CXCL12, CXCL12/SDF-1, hIRH, hSDF 1, IRH, PBSF, SCYB12, SDF 1, SDF 1a, SDF 1b, SDF1, SDF-1, SDF1A, SDF1B, Stromal cell derived factor 1, TLSF a, TLSF b, TPAR1
计算分子量 89 aa, 10 kDa
GenBank蛋白编号BC039893
基因名称 CXCL12/SDF-1
Gene ID (NCBI) 6387
RRIDAB_3672674
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP48061
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

In adulthood, CXCL12 plays an important role in angiogenesis by recruiting endothelial progenitor cells (EPCs) from the bone marrow through a CXCR4 dependent mechanism (PMID: 17878755). It is this function of CXCL12 that makes it a very important factor in carcinogenesis and the neovascularisation linked to tumour progression (PMID: 16943240). CXCL12 also has a role in tumor metastasis where cancer cells that express the receptor CXCR4 are attracted to metastasis target tissues that release the ligand, CXCL12 (PMID: 11242036 ). In breast cancer, however, increased expression of CXCL12 determines a reduced risk of distant metastasis (PMID: 19646861; 17724466 ). This antibody can detect all the isoforms of CXCL12/SDF1.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 CXCL12/SDF-1 antibody CL488-17402Download protocol
Standard Protocols
Click here to view our Standard Protocols