验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, K-562 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31700-1-AP targets CWF19L1 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag36356 Product name: Recombinant human CWF19L1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 325-538 aa of BC008746 Sequence: PGPCWFCLASPEVEKHLVVNIGTHCYLALAKGGLSDDHVLILPIGHYQSVVELSAEVVEEVEKYKATLRRFFKSRGKWCVVFERNYKSHHLQLQVIPVPISCSTTDDIKDAFITQAQEQQIELLEIPEHSDIKQIAQPGAAYFYVELDTGEKLFHRIKKNFPLQFGREVLASEAILNVPDKSDWRQCQISKEDEETLARRFRKDFEPYDFTLDD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CWF19-like 1, cell cycle control (S. pombe) |
| 别名 | SCAR17, hDrn1, C19L1 |
| 观测分子量 | 60-65 kDa |
| GenBank蛋白编号 | BC008746 |
| 基因名称 | CWF19L1 |
| Gene ID (NCBI) | 55280 |
| RRID | AB_3670086 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q69YN2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CWF19L1 belongs to the CWF19 family. CWF19L1 includes a metallophosphatase (MMP) domain, a histidine triad motif (HIT) domain, and a C-terminal Cwf-J-C domain. CWF19L1 is believed to be involved in cell cycle control, endosomal trafficking, and mRNA processing. CWF19L1 is a rare cause of autosomal recessive cerebellar ataxias (ARCA) (PMID: 36357319, PMID: 36453471). CWF19L1 is expressed in many brain regions, including cerebellum, thalamus and occipital, parietal and temporal lobes. CWF19L1 is also expressed in the spinal cord (PMID: 25361784).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CWF19L1 antibody 31700-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
