CWC15 Polyclonal antibody
CWC15 Polyclonal Antibody for IF/ICC, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC, IHC, IP, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse liver tissue, HeLa cells, HepG2 cells, Jurkat cells, mouse heart tissue, mouse testis tissue, PC-3 cells, SW 1990 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human stomach tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
The immunogen of 25293-1-AP is CWC15 Fusion Protein expressed in E. coli.
Tested Applications | IF/ICC, IHC, IP, WB,ELISA |
Cited Applications | WB |
Tested Reactivity | human, mouse |
Immunogen | CWC15 fusion protein Ag18116 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | CWC15 spliceosome-associated protein homolog (S. cerevisiae) |
Synonyms | C11orf5, CWC15, Cwf15, HSPC148, ORF5, Protein CWC15 homolog |
Calculated Molecular Weight | 229 aa, 27 kDa |
Observed Molecular Weight | 27-35 kDa |
GenBank Accession Number | BC040946 |
Gene Symbol | CWC15 |
Gene ID (NCBI) | 51503 |
RRID | AB_2880012 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9P013 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for CWC15 antibody 25293-1-AP | Download protocol |
IHC protocol for CWC15 antibody 25293-1-AP | Download protocol |
IF protocol for CWC15 antibody 25293-1-AP | Download protocol |
IP protocol for CWC15 antibody 25293-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nucleic Acids Res CTNNBL1 facilitates the association of CWC15 with CDC5L and is required to maintain the abundance of the Prp19 spliceosomal complex. |