验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, HeLa cells, HepG2 cells, Jurkat cells, mouse heart tissue, mouse testis tissue, PC-3 cells, SW 1990 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human stomach tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
25293-1-AP targets CWC15 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag18116 Product name: Recombinant human CWC15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-229 aa of BC040946 Sequence: MTTAARPTFEPARGGRGKGEGDLSQLSKQYSSRDLPSHTKIKYRQTTQDAPEEVRNRDFRRELEERERAAAREKNRDRPTREHTTSSSVSKKPRLDQIPAANLDADDPLTDEEDEDFEEESDDDDTAALLAELEKIKKERAEEQARKEQEQKAEEERIRMENILSGNPLLNLTGPSQPQANFKVKRRWDDDVVFKNCAKGVDDQKKDKRFVNDTLRSEFHKKFMEKYIK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | CWC15 spliceosome-associated protein homolog (S. cerevisiae) |
| 别名 | Protein CWC15 homolog, ORF5, HSPC148, Cwf15, C11orf5 |
| 计算分子量 | 229 aa, 27 kDa |
| 观测分子量 | 27-35 kDa |
| GenBank蛋白编号 | BC040946 |
| 基因名称 | CWC15 |
| Gene ID (NCBI) | 51503 |
| RRID | AB_2880012 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9P013 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CWC15, also named as C11orf5 or Spliceosome-associated protein CWC15 homolog, is a 229 amino acid protein, which belongs to the CWC15 family. CWC15 as a Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CWC15 antibody 25293-1-AP | Download protocol |
| IHC protocol for CWC15 antibody 25293-1-AP | Download protocol |
| IP protocol for CWC15 antibody 25293-1-AP | Download protocol |
| WB protocol for CWC15 antibody 25293-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
