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验证数据展示

  • IF Staining of mouse cortex using 11733-1-AP (same clone as 11733-1-PBS)

    IF Staining of mouse cortex using 11733-1-AP (same clone as 11733-1-PBS)

    IF result of the antibody CUX1 (11733-1-AP, 1:300) with mouse cortex, Data from Dr. Lee O. Vaasjo Muñoz, University of Chicago. . This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

  • WB analysis using 11733-1-AP (same clone as 11733-1-PBS)

    WB analysis using 11733-1-AP (same clone as 11733-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11733-1-AP (CUX1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 11733-1-AP (same clone as 11733-1-PBS)

    IHC staining of mouse brain using 11733-1-AP (same clone as 11733-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CUX1 antibody included
  • IHC staining of mouse brain using 11733-1-AP (same clone as 11733-1-PBS)

    IHC staining of mouse brain using 11733-1-AP (same clone as 11733-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11733-1-AP (CUX1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CUX1 antibody included
  • IP experiment of mouse brain using 11733-1-AP (same clone as 11733-1-PBS)

    IP experiment of mouse brain using 11733-1-AP (same clone as 11733-1-PBS)

    IP result of anti-CUX1 (IP:11733-1-AP, 4ug; Detection:11733-1-AP 1:1000) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 11733-1-AP (same clone as 11733-1-PBS)

    IF Staining of SH-SY5Y using 11733-1-AP (same clone as 11733-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using CUX1 antibody (11733-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11733-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CUX1 Polyclonal antibody, PBS Only

CUX1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11733-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

COY1, Clox, CDP1, CDP/Cut, CDP

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CUX1 Ready-To-Use IHC Kit. CUX1 primary antibody included.

产品信息

11733-1-PBS targets CUX1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2335

Product name: Recombinant human CUX1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-341 aa of BC025422

Sequence: MAANVGSMFQYWKRFDLQQLQRELDATATVLANRQDESEQSRKRLIEQSREFKKNTPEDLRKQVAPLLKSFQGEIDALSKRSKEAEAAFLNVYKRLIDVPDPVPALDLGQQLQLKVQRLHDIETENQKLRETLEEYNKEFAEVKNQEVTIKALKEKIREYEQTLKNQAETIALEKEQKLQNDFAEKERKLQETQMSTTSKLEEAEHKVQSLQTALEKTRTELFDLKTKYDEETTAKADEIEMIMTDLERANQRAEVAQREAETLREQLSSANHSLQLASQIQKAPDVAIEVLTRSSLEVELAAKEREIAQLVEDVQRLQASLTKLRENSASQISQLEQQLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cut-like homeobox 1
别名 COY1, Clox, CDP1, CDP/Cut, CDP
计算分子量 676 aa, 77 kDa
观测分子量 80 kDa
GenBank蛋白编号BC025422
基因名称 CUX1
Gene ID (NCBI) 1523
RRIDAB_2086995
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13948
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CUX1 is a transcription factors that regulates a large number of genes and microRNAs involved in multiple cellular processes, such as DNA replication, progression into S phase and later, the spindle assembly checkpoint that controls progression through mitosis. Cux1, a marker of supragranular layers. CUX1 acts by preventing binding of positively-acting CCAAT factors to promoters to mediated its gene reperssion. Also it's a component of nf-munr repressor, and binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. It can represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. CUX1 protein containing three cut repeats and a homeodomain called p200, there are several truncated isoforms that have been identified. These isoforms include a testis-specific isoform containing one cut repeat and the homeodomain called p55. In situ hybridization showed that mRNA for p55 was most abundant in round spermatids. Additional isoforms include p75, a protein similar in structure to p55, derived from the use of an alternate promoter in intron 20 ; the cut alternately spliced protein (CASP), a Golgi protein that contains amino-terminal sequences but none of the cut repeats or homeodomains ; and several other isoforms (p80, p90, p110, and p150) that appear to arise via proteolytic processing ]. This antibody can recognizes full-length p200 Cux1 and CASP protein (70~80kDa).

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