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验证数据展示

  • WB analysis of HEK-293 using 67034-1-Ig (same clone as 67034-1-PBS)

    WB analysis of HEK-293 using 67034-1-Ig (same clone as 67034-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67034-1-Ig (CUL7 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67034-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 67034-1-Ig (same clone as 67034-1-PBS)

    IHC staining of human heart using 67034-1-Ig (same clone as 67034-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67034-1-Ig (CUL7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67034-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 67034-1-Ig (same clone as 67034-1-PBS)

    IHC staining of human heart using 67034-1-Ig (same clone as 67034-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 67034-1-Ig (CUL7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67034-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 67034-1-Ig (same clone as 67034-1-PBS)

    WB analysis of HEK-293 using 67034-1-Ig (same clone as 67034-1-PBS)

    WB result of CUL7 antibody (67034-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL7 transfected HEK-293 cells. This data was developed using the same antibody clone with 67034-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CUL7 Monoclonal antibody, PBS Only

CUL7 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67034-1-PBS

产品说明书

CloneNo. 2E3G9

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat

应用

WB, IHC, Indirect ELISA

别名

CUL 7, CUL7, cullin 7, dJ20C7.5, KIAA0076

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

67034-1-PBS targets CUL7 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 Human, Mouse, Rat
免疫原

CatNo: Ag6943

Product name: Recombinant human CUL7 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1348-1698 aa of BC033647

Sequence: GKEHKSEKEEEAGAAAVVDVAEGEEEEEENEDLYYEGAMPEVSVLVLSRHSWPVASICHTLNPRTCLPSYLRGTLNRYSNFYNKSQSHPALERGSQRRLQWTWLGWAELQFGNQTLHVSTVQMWLLLYLNDLKAVSVESLLAFSGLSADMLNQAIGPLTSSRGPLDLHEQKDIPGGVLKIRDGSKEPRSRWDIVRLIPPQTYLQAEGEDGQNLEKRRNLLNCLIVRILKAHGDEGLHIDQLVCLVLEAWQKGPCPPRGLVSSLGKGSACSSTDVLSCILHLLGKGTLRRHDDRPQVLSYAVPVTVMEPHTESLNPGSSGPNPPLTFHTLQIRSRGVPYASCTATQSFSTFR

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 cullin 7
别名 CUL 7, CUL7, cullin 7, dJ20C7.5, KIAA0076
计算分子量 1698 aa, 191 kDa
观测分子量 185 kDa
GenBank蛋白编号BC033647
基因名称 CUL7
Gene ID (NCBI) 9820
RRIDAB_2882349
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ14999
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

he cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality. CUL7 was originally discovered as 185-kDa protein associated with the large T antigen of simian virus 40 (SV40). CUL7-deficient mice exhibit neonatal lethality with reduced size and vascular defects. CUL7 presumably plays a role in the DNA damage response by limiting p53 activity. CUL7 mutations have also been identified in 3-Msyndrome and the Yakuts short stature syndrome, both of which are characterized by pre- and post-natal growth retardation but with relatively normal mental and endocrine functions, suggesting that CUL7 may also be crucial for human placental development.