CoraLite®594-conjugated CUL1 Monoclonal antibody
CUL1 Monoclonal Antibody for FC (Intra), IF/ICC
Host / Isotype
Mouse / IgG2b
Reactivity
Human, Mouse, Rat
Applications
FC (Intra), IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
1B6D10
验证数据展示
经过测试的应用
| Positive IF detected in | HEK-293 cells |
| Positive FC detected in | HEK-293 cells |
For other applications, we recommend the unconjugated version of this antibody, 66978-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-66978 targets CUL1 in IF, FC (Intra) applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | FC (Intra), IF/ICC |
| Tested Reactivity | Human, Mouse, Rat |
| Immunogen | CUL1 fusion protein Ag28399 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | cullin 1 |
| Synonyms | CUL 1, CUL1, cullin 1 |
| Calculated Molecular Weight | 90 kDa |
| Observed Molecular Weight | 85 kDa |
| GenBank Accession Number | BC034318 |
| Gene Symbol | CUL1 |
| Gene ID (NCBI) | 8454 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q13616 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 CUL1 antibody CL594-66978 | Download protocol |
| FC protocol for CL594 CUL1 antibody CL594-66978 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
