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Host
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Species Reactivity
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验证数据展示

  • WB analysis using 82474-1-RR (same clone as 82474-1-PBS)

    WB analysis using 82474-1-RR (same clone as 82474-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82474-1-RR (CUL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82474-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 82474-1-RR (same clone as 82474-1-PBS)

    WB analysis of A549 using 82474-1-RR (same clone as 82474-1-PBS)

    WB result of CUL1 antibody (82474-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL1 transfected A549 cells. This data was developed using the same antibody clone with 82474-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 82474-1-RR (same clone as 82474-1-PBS)

    IP experiment of HeLa using 82474-1-RR (same clone as 82474-1-PBS)

    IP result of anti-CUL1 (IP:82474-1-RR, 4ug; Detection:82474-1-RR 1:1000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 82474-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82474-1-RR

    Affinity and Kinetic Characterization of 82474-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82474-1-RR against Human CUL1 were performed. The affinity constant is 21.7 nM.

CUL1 Recombinant monoclonal antibody, PBS Only

CUL1 Uni-rAb® Recombinant Antibody for Indirect ELISA, IP, WB
Cat No. 82474-1-PBS

产品说明书

CloneNo. 5K17

宿主/亚型

Rabbit / IgG

种属反应性

Human, rat, mouse

应用

Indirect ELISA, IP, WB

别名

Cullin-1, Cullin1, CUL-1, 5K17

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CUL1 Ready-To-Use IHC Kit. CUL1 primary antibody included.

产品信息

82474-1-PBS targets CUL1 in Indirect ELISA, IP, WB applications and shows reactivity with Human, rat, mouse samples.

经测试应用 Indirect ELISA, IP, WB Application Description
经测试反应性 Human, rat, mouse
免疫原

CatNo: Ag3585

Product name: Recombinant human CUL1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 111-462 aa of BC034318

Sequence: YCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQFCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQQLTDSTQIKMDILAQVLQILLKSKLLVLEDENANVDEVELKPDTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cullin 1
别名 Cullin-1, Cullin1, CUL-1, 5K17
计算分子量 90 kDa
观测分子量90 kDa
GenBank蛋白编号BC034318
基因名称 CUL1
Gene ID (NCBI) 8454
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13616
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

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