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Host
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验证数据展示

  • WB analysis using 82474-1-RR (same clone as 82474-1-PBS)

    WB analysis using 82474-1-RR (same clone as 82474-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82474-1-RR (CUL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82474-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 82474-1-RR (same clone as 82474-1-PBS)

    WB analysis of A549 using 82474-1-RR (same clone as 82474-1-PBS)

    WB result of CUL1 antibody (82474-1-RR; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL1 transfected A549 cells. This data was developed using the same antibody clone with 82474-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 82474-1-RR (same clone as 82474-1-PBS)

    IP experiment of HeLa using 82474-1-RR (same clone as 82474-1-PBS)

    IP result of anti-CUL1 (IP:82474-1-RR, 4ug; Detection:82474-1-RR 1:1000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 82474-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82474-1-RR

    Affinity and Kinetic Characterization of 82474-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82474-1-RR against Human CUL1 were performed. The affinity constant is 21.7 nM.

CUL1 Recombinant antibody, PBS Only

CUL1 Uni-rAbTM Recombinant Antibody for Indirect ELISA, IP, WB
Cat No. 82474-1-PBS

产品说明书

CloneNo. 5K17

宿主/亚型

Rabbit / IgG

种属反应性

Human, rat, mouse

应用

Indirect ELISA, IP, WB

别名

Cullin-1, Cullin1, CUL-1, 5K17

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CUL1 Ready-To-Use IHC Kit. CUL1 primary antibody included.

产品信息

82474-1-PBS targets CUL1 in Indirect ELISA, IP, WB applications and shows reactivity with Human, rat, mouse samples.

经测试应用 Indirect ELISA, IP, WB Application Description
经测试反应性 Human, rat, mouse
免疫原 CUL1 fusion protein Ag3585 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cullin 1
别名 Cullin-1, Cullin1, CUL-1, 5K17
计算分子量 90 kDa
观测分子量90 kDa
GenBank蛋白编号BC034318
基因名称 CUL1
Gene ID (NCBI) 8454
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13616
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.