验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, HEK-293 cells, MCF-7 cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:17000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
82474-1-RR targets CUL1 in WB, IP, ELISA applications and shows reactivity with Human, rat, mouse samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 经测试反应性 | Human, rat, mouse |
| 免疫原 |
CatNo: Ag3585 Product name: Recombinant human CUL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 111-462 aa of BC034318 Sequence: YCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQFCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQQLTDSTQIKMDILAQVLQILLKSKLLVLEDENANVDEVELKPDTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | cullin 1 |
| 别名 | Cullin-1, Cullin1, CUL-1, 5K17 |
| 计算分子量 | 90 kDa |
| 观测分子量 | 90 kDa |
| GenBank蛋白编号 | BC034318 |
| 基因名称 | CUL1 |
| Gene ID (NCBI) | 8454 |
| RRID | AB_3086481 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q13616 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases. Humans express seven cullin proeins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CUL1 antibody 82474-1-RR | Download protocol |
| IP protocol for CUL1 antibody 82474-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |