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验证数据展示

  • WB analysis using 11381-1-AP

    WB analysis using 11381-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11381-1-AP (Cortactin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 11381-1-AP

    WB analysis of HeLa using 11381-1-AP

    WB result of Cortactin antibody (11381-1-AP; 1:200000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Cortactin transfected HeLa cells.

  • IHC staining of human breast cancer using 11381-1-AP

    IHC staining of human breast cancer using 11381-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11381-1-AP (Cortactin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cortactin antibody included
  • IHC staining of human breast cancer using 11381-1-AP

    IHC staining of human breast cancer using 11381-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11381-1-AP (Cortactin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cortactin antibody included
  • IP experiment of MCF-7 using 11381-1-AP

    IP experiment of MCF-7 using 11381-1-AP

    IP result of anti-Cortactin (IP:11381-1-AP, 4ug; Detection:11381-1-AP 1:1000) with MCF-7 cells lysate 2400 ug.

  • WB analysis using 11381-1-AP

    WB analysis using 11381-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11381-1-AP (Cortactin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of HAP1 using 11381-1-AP

    WB analysis of HAP1 using 11381-1-AP

    N/A

  • IHC staining of mouse colon using 11381-1-AP

    IHC staining of mouse colon using 11381-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11381-1-AP (Cortactin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cortactin antibody included
  • IHC staining of mouse colon using 11381-1-AP

    IHC staining of mouse colon using 11381-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11381-1-AP (Cortactin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Cortactin antibody included
  • IF Staining of HeLa using 11381-1-AP

    IF Staining of HeLa using 11381-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cortactin antibody (11381-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HeLa using 11381-1-AP

    FC experiment of HeLa using 11381-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cortactin Polyclonal antibody (11381-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Featured Product
  • KD/KO Validated

Cortactin Polyclonal antibody

Cortactin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 11381-1-AP

产品说明书

引用文献(30)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

别名

CTTN, EMS1, SRC8

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HAP1 cells, HeLa cells, HUVEC cells, HEK-293 cells, HT-1080 cells, MCF-7 cells, NIH/3T3 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue, mouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Cortactin Ready-To-Use IHC Kit. Cortactin primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11381-1-AP targets Cortactin in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag1940

Product name: Recombinant human Cortactin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 206-513 aa of BC008799

Sequence: VDKSAVGFEYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKCALGWDHQEKLQLHESQKDYSKGFGGKYGVQKDRMDKNASTFEDVTQVSSAYQKTVPVEAVTSKTSNIRANFENLAKEKEQEDRRKAEAERAQRMAKERQEQEEARRKLEEQARAKTQTPPVSPAPQPTEERLPSSPVYEDAASFKAELSYRGPVSGTEPEPVYSMEAADYREASSQQGLAYATEAVYESAEAPGHYPAEDSTYDEYENDLGITAVALYDYQAAGDDEISFDPDDIITNIEMIDDGWWRGVCKGRYGLFPANYVELRQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cortactin
别名 CTTN, EMS1, SRC8
计算分子量 57 kDa
观测分子量70-85 kDa
GenBank蛋白编号BC008799
基因名称 Cortactin
Gene ID (NCBI) 2017
RRIDAB_2088125
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14247
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cortactin, encoded by CTTN gene (formerly EMS1), was originally identified as a substrate of the Src kinase. It is a cytoskeletal protein that interacts with F-actin and Arp2/3 complex and plays an important role in regulation of actin assembly and cytoskeleton dynamics. Overexpression of cortactin increases cell migration, and is thought to contribute to the invasive phenotype of breast cancer and several other tumors. Three isoforms of cortactin exist due to the alternative splicing. Although cortactin has a predicted molecular weight around 65 kDa, it was detected as 80/85 doublet or as multiple bands between 75 and 85 kDa on SDS-PAGE (PMID: 18607838, 19089942).

实验方案

Product Specific Protocols
FC protocol for Cortactin antibody 11381-1-APDownload protocol
IF protocol for Cortactin antibody 11381-1-APDownload protocol
IHC protocol for Cortactin antibody 11381-1-APDownload protocol
IP protocol for Cortactin antibody 11381-1-APDownload protocol
WB protocol for Cortactin antibody 11381-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Host Microbe

Lactate production by tumor-resident Staphylococcus promotes metastatic colonization in lung adenocarcinoma

Authors - Huan Yu
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mouseIF

Circ. Res.

Inhibition of KLF5-Myo9b-RhoA Pathway-Mediated Podosome Formation in Macrophages Ameliorates Abdominal Aortic Aneurysm.

Authors - Dong Ma
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humanWB

Mol Cell

Spliceosomal proofreading factors safeguard 3' splice-site fidelity and prevent proteotoxicity and inflammation

Authors - Fangshu Li
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humanWB

J Exp Clin Cancer Res

Tumor cell-derived SPON2 promotes M2-polarized tumor-associated macrophage infiltration and cancer progression by activating PYK2 in CRC.

Authors - Chengmei Huang
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humanIF

Cell Death Dis

ACAT2 suppresses the ubiquitination of YAP1 to enhance the proliferation and metastasis ability of gastric cancer via the upregulation of SETD7

Authors - Mengmeng Zhang
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humanIHC

Cancer Lett

Sulforaphane suppresses bladder cancer metastasis via blocking actin nucleation-mediated pseudopodia formation

Authors - Lei Huang
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