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验证数据展示

  • WB analysis using 27538-1-AP

    WB analysis using 27538-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27538-1-AP (CTSS antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 27538-1-AP

    IHC staining of human colon cancer using 27538-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27538-1-AP (CTSS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 27538-1-AP

    IHC staining of human colon cancer using 27538-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27538-1-AP (CTSS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of U-87 MG using 27538-1-AP

    IF Staining of U-87 MG using 27538-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-87 MG cells using CTSS antibody (27538-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

CTSS Polyclonal antibody

CTSS Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 27538-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

EC:3.4.22.27, Cathepsin S

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHUVEC cells, THP-1 cells, U-87 MG cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU-87 MG cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27538-1-AP targets CTSS in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CTSS fusion protein Ag25881 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cathepsin S
别名 EC:3.4.22.27, Cathepsin S
计算分子量 37 kDa
观测分子量 25 kDa, 37 kDa
GenBank蛋白编号BC002642
基因名称 CTSS
Gene ID (NCBI) 1520
RRIDAB_3085966
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP25774
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cathepsin S (CTSS) is a cysteine protease that plays an important role in extracellular matrix degradation and remodeling, antigen presentation, inflammation, and angiogenesis; it has been identified as a radiation response gene. In malignant tumors, CTSS induces tumor proliferation, invasion and metastasis through various mechanisms. CTSS proteins could be detected in two forms, pro-CTSS (35-37 kDa) and active CTSS (25 kDa) (PMID: 30006610, PMID: 34208434). And in this publication, it was also reporeted that active CTSS was expressed at higher levels than pro-CTSS in the lysate (PMID: 34208434).

实验方案

Product Specific Protocols
WB protocol for CTSS antibody 27538-1-APDownload protocol
IHC protocol for CTSS antibody 27538-1-APDownload protocol
IF protocol for CTSS antibody 27538-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols