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验证数据展示

  • WB analysis using 84572-4-RR

    WB analysis using 84572-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84572-4-RR (CTRL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse pancreas using 84572-4-RR

    WB analysis of mouse pancreas using 84572-4-RR

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 84572-4-RR (CTRL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse pancreas using 84572-4-RR

    IHC staining of mouse pancreas using 84572-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84572-4-RR (CTRL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse pancreas using 84572-4-RR

    IHC staining of mouse pancreas using 84572-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84572-4-RR (CTRL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CTRL Recombinant antibody

CTRL Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 84572-4-RR

产品说明书

CloneNo. 241858D7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

241858D7, chymotrypsin like, Chymotrypsin-like protease CTRL-1, CTRL1, EC:3.4.21.-

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse stomach tissue, mouse pancreas tissue, rat pancreas tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84572-4-RR targets CTRL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CTRL fusion protein Eg2214 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chymotrypsin-like
别名 241858D7, chymotrypsin like, Chymotrypsin-like protease CTRL-1, CTRL1, EC:3.4.21.-
计算分子量 28 kDa
观测分子量 25-28 kDa
GenBank蛋白编号BC063475
基因名称 CTRL
Gene ID (NCBI) 1506
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP40313
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CTRL(Chymotrypsin-like protease) is also named as CTRL1 and belongs to the peptidase S1 family. The CTRL1 enzyme is active over a broad pH range. It expresses in pancreas and presence in intestinal fluid after stimulation of pancreatic secretion as a digestive enzyme(PMID:9065485). This gene encode a protein of 264 amino acid and the protein has a signal peptide of 18 amino acid and a propeptide of 15 amino acid.

实验方案

Product Specific Protocols
WB protocol for CTRL antibody 84572-4-RRDownload protocol
IHC protocol for CTRL antibody 84572-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols