×
Use Able AI chat for product recommendations

验证数据展示

  • WB analysis of A549 using 66208-1-Ig

    WB analysis of A549 using 66208-1-Ig

    A549 cells were subjected to SDS PAGE followed by western blot with 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of fetal human brain using 66208-1-Ig

    WB analysis of fetal human brain using 66208-1-Ig

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66208-1-Ig (p120 Catenin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IF Staining of human breast cancer using 66208-1-Ig

    IF Staining of human breast cancer using 66208-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of MCF-7 using 66208-1-Ig

    IF Staining of MCF-7 using 66208-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using p120 Catenin antibody (66208-1-Ig, Clone: 2F7H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

  • IHC staining of human breast cancer using 66208-1-Ig

    IHC staining of human breast cancer using 66208-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with p120 Catenin antibody included
  • IHC staining of human breast cancer using 66208-1-Ig

    IHC staining of human breast cancer using 66208-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with p120 Catenin antibody included
  • IHC staining of human liver using 66208-1-Ig

    IHC staining of human liver using 66208-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human ovary cancer using 66208-1-Ig

    IHC staining of human ovary cancer using 66208-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human breast cancer using 66208-1-Ig

    IHC staining of human breast cancer using 66208-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer.

  • IHC staining of human breast cancer using 66208-1-Ig

    IHC staining of human breast cancer using 66208-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with p120 Catenin antibody included
  • IHC staining of human breast cancer using 66208-1-Ig

    IHC staining of human breast cancer using 66208-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with p120 Catenin antibody included
  • IHC staining of human colorectal cancer using 66208-1-Ig

    IHC staining of human colorectal cancer using 66208-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colorectal cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with p120 Catenin antibody included
  • IHC staining of human stomach cancer using 66208-1-Ig

    IHC staining of human stomach cancer using 66208-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with p120 Catenin antibody included
  • IHC staining of human stomach cancer using 66208-1-Ig

    IHC staining of human stomach cancer using 66208-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with p120 Catenin antibody included
  • IHC staining of human ovary cancer using 66208-1-Ig

    IHC staining of human ovary cancer using 66208-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with p120 Catenin antibody included
  • IHC staining of human liver using 66208-1-Ig

    IHC staining of human liver using 66208-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with p120 Catenin antibody included
  • IHC staining of human breast cancer using 66208-1-Ig

    IHC staining of human breast cancer using 66208-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with p120 Catenin antibody included
  • IHC staining of human breast cancer using 66208-1-Ig

    IHC staining of human breast cancer using 66208-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with p120 Catenin antibody included
  • IHC staining of human colon using 66208-1-Ig

    IHC staining of human colon using 66208-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with p120 Catenin antibody included
  • IHC staining of human breast using 66208-1-Ig

    IHC staining of human breast using 66208-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with p120 Catenin antibody included
  • IHC staining of human appendicitis using 66208-1-Ig

    IHC staining of human appendicitis using 66208-1-Ig

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with p120 Catenin antibody included
  • Featured Product
  • KD/KO Validated

p120 Catenin Monoclonal antibody

p120 Catenin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66208-1-Ig

产品说明书

CloneNo. 2F7H8
引用文献(9)

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (2)

别名

CTNND1, Cadherin-associated Src substrate, CAS, Catenin delta 1, Catenin delta-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inA549 cells, fetal human brain tissue, HEK-293 cells, HeLa cells, mouse brain tissue, A431 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue, mouse colon tissue, rat colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells, HeLa cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy p120 Catenin Ready-To-Use IHC Kit. p120 Catenin primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66208-1-Ig targets p120 Catenin in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, canine
免疫原

CatNo: Ag2824

Product name: Recombinant human CTNND1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 546-830 aa of BC010501

Sequence: GYELLFQPEVVRIYISLLKESKTPAILEASAGAIQNLCAGRWTYGRYIRSALRQEKALSAIADLLTNEHERVVKAASGALRNLAVDARNKELIGKHAIPNLVKNLPGGQQNSSWNFSEDTVISILNTINEVIAENLEAAKKLRETQGIEKLVLINKSGNRSEKEVRAAALVLQTIWGYKELRKPLEKEGWKKSDFQVNLNNASRSQSSHSYDDSTLPLIDRNQKSDKKPDREEIQMSNMGSNTKSLDNNYSTPNERGDHNKTLDRSGDLGDMEPLKGTTPLMQKI

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 catenin (cadherin-associated protein), delta 1
别名 CTNND1, Cadherin-associated Src substrate, CAS, Catenin delta 1, Catenin delta-1
计算分子量 948 aa, 105 kDa
观测分子量 90-120 kDa
GenBank蛋白编号BC010501
基因名称 p120 Catenin
Gene ID (NCBI) 1500
RRIDAB_2881599
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO60716
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Catenins were discovered as proteins that are linked to the cytoplasmic domain of transmembrane cadherins (PMID: 9653641). p120 catenin, also called p120 ctn or catenin delta-1, regulates cell-cell adhesion through its interaction with the cytoplasmic tail of classical and type II cadherins. p120 catenin is a tyrosine kinase substrate implicated in cell transformation by SRC, as well as in ligand-induced receptor signaling through the EGF receptor, the PDGF receptor, and the CSF1 receptor. Different expression patterns of p120 catenin in lobular and ductal carcinomas of breast have been reported: membrane stain for ductal carcinoma and cytoplasmic stain for lobular carcinoma (PMID: 24966968). Different isoforms of p120 catenin are variably expressed in different tissues as a result of alternative splicing and the use of multiple translation initiation codons (PMID: 19150613).

实验方案

Product Specific Protocols
IF protocol for p120 Catenin antibody 66208-1-IgDownload protocol
IHC protocol for p120 Catenin antibody 66208-1-IgDownload protocol
WB protocol for p120 Catenin antibody 66208-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP,IF

Mol Oncol

Kaiso phosphorylation at threonine 606 leads to its accumulation in the cytoplasm, reducing its transcriptional repression of the tumour suppressor CDH1.

Authors - Wei Tian
View Article
mouseIF

Development

Ontogenesis of the tear drainage system requires Prickle1-driven polarized basement membrane deposition.

Authors - Dianlei Guo
View Article
humanIP

Proc Natl Acad Sci U S A

SENCR stabilizes vascular endothelial cell adherens junctions through interaction with CKAP4.

Authors - Qing Lyu
View Article
ratWB,IF

Biomed Pharmacother

Inhibition of autophagy and RIP1/RIP3/MLKL-mediated necroptosis by edaravone attenuates blood spinal cord barrier disruption following spinal cord injury

Authors - Bo Xu
View Article
humanWB

Clin Exp Pharmacol Physiol

Colorectal cancer cells with stably expressed SIRT3 demonstrate proliferating retardation by Wnt/β-catenin cascade inactivation

Authors - Tianyu Li
View Article

Cancer Discov

Spatial Transcriptomics of Intraductal Papillary Mucinous Neoplasms of the Pancreas Identifies NKX6-2 as a Driver of Gastric Differentiation and Indolent Biological Potential

Authors - Marta Sans
View Article
||
New chat

Able™

正在加载，请稍候...