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验证数据展示

  • WB analysis of fetal human brain using 66208-1-Ig (same clone as 66208-1-PBS)

    WB analysis of fetal human brain using 66208-1-Ig (same clone as 66208-1-PBS)

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66208-1-Ig (p120 Catenin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • IF Staining of human breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

    IF Staining of human breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 66208-1-Ig (same clone as 66208-1-PBS)

    IF Staining of A431 using 66208-1-Ig (same clone as 66208-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using p120 Catenin antibody (66208-1-Ig, Clone: 2F7H8 ) at dilution of 1:800 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

    IHC staining of human breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with p120 Catenin antibody included
  • IHC staining of human breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

    IHC staining of human breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with p120 Catenin antibody included
  • IHC staining of human breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

    IHC staining of human breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66208-1-Ig (p120 Catenin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with p120 Catenin antibody included
  • IF Staining of mouse breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

    IF Staining of mouse breast cancer using 66208-1-Ig (same clone as 66208-1-PBS)

    Immunofluorescent analysis of un-fixed frozen OCT-embedded mouse breast cancer using p120 Catenin antibody (66208-1-Ig, Clone: 2F7H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66208-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

p120 Catenin Monoclonal antibody, PBS Only

p120 Catenin Monoclonal Antibody for WB, IHC, IF/ICC, IF-Fro, Indirect ELISA
Cat No. 66208-1-PBS

产品说明书

CloneNo. 2F7H8

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-Fro, Indirect ELISA

别名

CTNND1, Cadherin-associated Src substrate, CAS, Catenin delta 1, Catenin delta-1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy p120 Catenin Ready-To-Use IHC Kit. p120 Catenin primary antibody included.

产品信息

66208-1-PBS targets p120 Catenin in WB, IHC, IF/ICC, IF-Fro, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-Fro, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2824

Product name: Recombinant human CTNND1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 546-830 aa of BC010501

Sequence: GYELLFQPEVVRIYISLLKESKTPAILEASAGAIQNLCAGRWTYGRYIRSALRQEKALSAIADLLTNEHERVVKAASGALRNLAVDARNKELIGKHAIPNLVKNLPGGQQNSSWNFSEDTVISILNTINEVIAENLEAAKKLRETQGIEKLVLINKSGNRSEKEVRAAALVLQTIWGYKELRKPLEKEGWKKSDFQVNLNNASRSQSSHSYDDSTLPLIDRNQKSDKKPDREEIQMSNMGSNTKSLDNNYSTPNERGDHNKTLDRSGDLGDMEPLKGTTPLMQKI

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 catenin (cadherin-associated protein), delta 1
别名 CTNND1, Cadherin-associated Src substrate, CAS, Catenin delta 1, Catenin delta-1
计算分子量 948 aa, 105 kDa
观测分子量 90-120 kDa
GenBank蛋白编号BC010501
基因名称 p120 Catenin
Gene ID (NCBI) 1500
RRIDAB_2881599
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO60716
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Catenins were discovered as proteins that are linked to the cytoplasmic domain of transmembrane cadherins (PMID: 9653641). p120 catenin, also called p120 ctn or catenin delta-1, regulates cell-cell adhesion through its interaction with the cytoplasmic tail of classical and type II cadherins. p120 catenin is a tyrosine kinase substrate implicated in cell transformation by SRC, as well as in ligand-induced receptor signaling through the EGF receptor, the PDGF receptor, and the CSF1 receptor. Different expression patterns of p120 catenin in lobular and ductal carcinomas of breast have been reported: membrane stain for ductal carcinoma and cytoplasmic stain for lobular carcinoma (PMID: 24966968). Different isoforms of p120 catenin are variably expressed in different tissues as a result of alternative splicing and the use of multiple translation initiation codons (PMID: 19150613).

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