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验证数据展示

  • WB analysis using 87022-1-RR

    WB analysis using 87022-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 87022-1-RR (CTNNA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat heart using 87022-1-RR

    IHC staining of rat heart using 87022-1-RR

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 87022-1-RR (CTNNA3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CTNNA3 antibody included
  • IF Staining of rat heart using 87022-1-RR

    IF Staining of rat heart using 87022-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using CTNNA3 antibody (87022-1-RR, Clone: 252141F2, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8, red ) at dilution of 1:200 and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of rat heart using 87022-1-RR

    IF Staining of rat heart using 87022-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using CTNNA3 antibody (87022-1-RR, Clone: 252141F2, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8, red ) at dilution of 1:200 and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse heart using 87022-1-RR

    IF Staining of mouse heart using 87022-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using CTNNA3 antibody (87022-1-RR, Clone: 252141F2, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8, red ) at dilution of 1:200 and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse heart using 87022-1-RR

    IF Staining of mouse heart using 87022-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat heart tissue using CTNNA3 antibody (87022-1-RR, Clone: 252141F2, green) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cardiac Troponin T antibody (68300-1-Ig, Clone: 4E2D8, red ) at dilution of 1:200 and Multi-rAb® CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 87022-1-RR

    Affinity and Kinetic Characterization of 87022-1-RR

    Biolayer interferometry (BLl) kinetic assays of 87022-1-RR against Human CTNNA3 were performed. The affinity constant is 3.11 nM.

CTNNA3 Recombinant monoclonal antibody

CTNNA3 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 87022-1-RR

产品说明书

CloneNo. 252141F2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, canine

应用

WB, IHC, IF-P, ELISA

别名

Alpha T catenin, Alpha T-catenin, Cadherin-associated protein, Catenin alpha 3, Catenin alpha-3

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, MDCK cells, C2C12 cells, C6 cells, PC-12 cells, mouse testis tissue, rat brain tissue
Positive IHC detected inrat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat heart tissue, mouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy CTNNA3 Ready-To-Use IHC Kit. CTNNA3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

87022-1-RR targets CTNNA3 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat, canine
免疫原

CatNo: Ag5008

Product name: Recombinant human CTNNA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-376 aa of BC030646

Sequence: MSAETPITLNIDPQDLQVQTFTVEKLLEPLIIQVTTLVNCPQNPSSRKKGRSKRASVLLASVEEATWNLLDKGEKIAQEATVLKDELTASLEKVRKESEALKVSAERFTDDPCFLPKREAVVQAARALLAAVTRLLILADMIDVMCLLQHVSAFQRTFESLKNVANKSDLQKTYQKLGKELENLDYLAFKRQQDLKSPNQRDEIAGARASLKENSPLLHSICSACLEHSDVASLKASKDTVCEEIQNALNVISNASQGIQNMTTPPEPQAATLGSALDELENLIVLNPLTVTEEEIRPSLEKRLEAIISGAALLADSSCTRDLHRERIIAECNAIRQALQDLLSEYMNNLCNQKSDLGLTGVKIKLLEECIIFWSL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 catenin (cadherin-associated protein), alpha 3
别名 Alpha T catenin, Alpha T-catenin, Cadherin-associated protein, Catenin alpha 3, Catenin alpha-3
计算分子量 100 kDa
观测分子量 100 kDa
GenBank蛋白编号BC030646
基因名称 CTNNA3
Gene ID (NCBI) 29119
RRIDAB_3745280
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9UI47
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha catenin is an essential component of adherens junctions that connects E-cadherin-β-catenin complexes with the actin cytoskeleton. The alpha catenin family of proteins consists of three members, alpha-E-catenin (CTNNA1), alpha-N-catenin (CTNNA2), and alpha-T-catenin (CTNNA3)(PMID: 21598020). CTNNA3 is a cell adhesion molecule. In intercalated discs of the heart, CTNNA3 is a component of a unique hybrid adhering junction, or area composita, that contains proteins associated with both desmosomes and adherens junctions (PMID: 22421363). Mutations in CTNNA3 are associated with arrhythmogenic right ventricular dysplasia, familial 13(PMID: 23136403).

实验方案

Product Specific Protocols
IF protocol for CTNNA3 antibody 87022-1-RRDownload protocol
IHC protocol for CTNNA3 antibody 87022-1-RRDownload protocol
WB protocol for CTNNA3 antibody 87022-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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