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验证数据展示

  • FC experiment of mouse splenocytes using Biotin-FcA98147

    FC experiment of mouse splenocytes using Biotin-FcA98147

    1x10^6 untreated or Con A-treated mouse splenocytes were surface stained with 0.25 ug Biotin Anti-Mouse CTLA-4/CD152 Rabbit RecAb (Biotin-FcA98147, Clone: 241100B8) and Streptavidin-PE Conjugate (PE-PF00030). Cells were co-stained with FcZero-rAb® APC Anti-Mouse CD4 (RM4-4) Rabbit Recombinant Antibody (APC-FcA65655, Clone: RM4-4). Cells were not fixed.

  • FC experiment of mouse splenocytes using Biotin-FcA98147

    FC experiment of mouse splenocytes using Biotin-FcA98147

    1x10^6 Con A-treated mouse splenocytes were surface stained with 0.25 ug Biotin Anti-Mouse CTLA-4/CD152 Rabbit RecAb (Biotin-FcA98147, Clone: 241100B8) (red) or FcZero-rAb® Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue), and Streptavidin-PE Conjugate (PE-PF00030). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Biotin Anti-Mouse CTLA-4/CD152 Rabbit RecAb (Biotin-FcA98147, Clone: 241100B8) (black, dashed) and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.

  • Zero Fc receptor binding
  • Site-specific conjugation
  • One universal isotype

FcZero-rAb® Biotin Anti-Mouse CTLA-4/CD152 Rabbit Recombinant Antibody

CTLA-4/CD152 FcZero-rAb® Recombinant Antibody for FC
Cat No. Biotin-FcA98147

产品说明书

CloneNo. 241100B8

宿主/亚型

Rabbit / IgG

种属反应性

mouse

应用

FC

别名

Ctla4, Cd152, Ctla 4, Ly 56

缓冲液配方:  PBS, Azide
PBS, Azide
偶联物:  Biotin
规格价格库存

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经过测试的应用

Positive FC detected inCon A treated mouse splenocytes

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

Biotin-FcA98147 targets CTLA-4/CD152 in FC applications and shows reactivity with mouse samples.

经测试应用 FC Application Description
经测试反应性 mouse
免疫原

CatNo: Eg0632

Product name: Recombinant Mouse CTLA-4 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 36-162 aa of NM_009843.4

Sequence: EAIQVTQPSVVLASSHGVASFPCEYSPSHNTDEVRVTVLRQTNDQMTEVCATTFTEKNTVGFLDYPFCSGTFNESRVNLTIQGLRAVDTGLYLCKVELMYPPPYFVGMGNGTQIYVIDPEPCPDSDF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytotoxic T-lymphocyte-associated protein 4
别名 Ctla4, Cd152, Ctla 4, Ly 56
计算分子量25 kDa
GenBank蛋白编号NM_009843.4
基因名称 CTLA-4
Gene ID (NCBI) 12477
RRIDAB_3746941
偶联类型 Biotin
形式Liquid
纯化方式Protein A purification
UNIPROT IDP09793
储存缓冲液 PBS with 0.09% sodium azide, pH 7.3.
储存条件Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses. Besides the full-length transmembrane form, CTLA-4 also exists in a truncated soluble form (sCTLA-4).

实验方案

Product Specific Protocols
FC protocol for Biotin CTLA-4/CD152 antibody Biotin-FcA98147Download protocol
Standard Protocols
Click here to view our Standard Protocols
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