CTLA-4 Polyclonal antibody
CTLA-4 Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Mouse
Applications
WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | Con A treated mouse splenocytes |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30648-1-AP targets CTLA-4 in WB, ELISA applications and shows reactivity with Mouse samples.
Tested Applications | WB, ELISA |
Tested Reactivity | Mouse |
Immunogen | Recombinant protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cytotoxic T-lymphocyte-associated protein 4 |
Synonyms | Cd152, Ctla 4, Ctla4, Ly 56 |
Calculated Molecular Weight | 25 kDa |
Observed Molecular Weight | 28-40 kDa |
GenBank Accession Number | NM_009843.4 |
Gene Symbol | Ctla4 |
Gene ID (NCBI) | 12477 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P09793 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses. Besides the full-length transmembrane form, CTLA-4 also exists in a truncated soluble form (sCTLA-4).
实验方案
Product Specific Protocols | |
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WB protocol for CTLA-4 antibody 30648-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |