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验证数据展示

  • IHC staining of human prostate cancer using 55279-1-AP

    IHC staining of human prostate cancer using 55279-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55279-1-AP (CTAGE5 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 55279-1-AP

    WB analysis using 55279-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55279-1-AP (CTAGE5 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 55279-1-AP

    WB analysis of HepG2 using 55279-1-AP

    WB result of CTAGE5 antibody (55279-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CTAGE5 transfected HepG2 cells.

  • IHC staining of human prostate cancer using 55279-1-AP

    IHC staining of human prostate cancer using 55279-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 55279-1-AP (CTAGE5 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

CTAGE5 Polyclonal antibody

CTAGE5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 55279-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

MEA6, MEA11, Cutaneous T-cell lymphoma-associated antigen 5, CTAGE family, member 5, CTAGE family member 5 ER export factor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293T cells, HepG2 cells, human brain tissue, PC-3 cells, human testis tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55279-1-AP targets CTAGE5 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CTAGE family, member 5
别名 MEA6, MEA11, Cutaneous T-cell lymphoma-associated antigen 5, CTAGE family, member 5, CTAGE family member 5 ER export factor
计算分子量 92 kDa
观测分子量 100-110 kDa
GenBank蛋白编号NM_005930
基因名称 CTAGE5
Gene ID (NCBI) 4253
RRIDAB_11183044
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96PC5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cutaneous T cell lymphoma-associated antigen 5 (CTAGE5) is a member of the cTAGE family. CTAGE5 is a tumor-associated antigen and is overexpressed in various cancer cell lines. CTAGE5 consists of a signal anchor motif, a single transmembrane domain, two coiled-coil motifs, and a proline-rich domain (PRD) from the N-terminus. CTAGE5 interacts with TANGO1 through its second coil region to form a cargo receptor complex (PMID: 21525241). This antibody is specific to CTAGE5. It can recognize all the isoforms except isoform 5B.

实验方案

Product Specific Protocols
WB protocol for CTAGE5 antibody 55279-1-APDownload protocol
IHC protocol for CTAGE5 antibody 55279-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols