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NY-ESO-1 Polyclonal antibody

NY-ESO-1 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 19521-1-AP

Print datasheet

Synonyms

Cancer/testis antigen 1, cancer/testis antigen 1A, Cancer/testis antigen 6.1, CT6.1, CTAG, CTAG1, CTAG1A, CTAG1B, ESO1, L antigen family member 2, LAGE 2, LAGE2, LAGE2A, LAGE2B, NY-ESO-1

验证数据展示

  • WB analysis of human testis using 19521-1-AP

    WB analysis of human testis using 19521-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 19521-1-AP (CTAG1A antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 19521-1-AP

    IF Staining of HepG2 using 19521-1-AP

    Immunofluorescent analysis of HepG2 cells, using CTAG1A antibody 19521-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of HepG2 using 19521-1-AP

    IP experiment of HepG2 using 19521-1-AP

    IP result of anti-NY-ESO-1 (IP:19521-1-AP, 4ug; Detection:19521-1-AP 1:500) with HepG2 cells lysate 3200ug.

  • IHC staining of mouse testis using 19521-1-AP

    IHC staining of mouse testis using 19521-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19521-1-AP (NY-ESO-1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse testis using 19521-1-AP

    IHC staining of mouse testis using 19521-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 19521-1-AP (NY-ESO-1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman testis tissue, HT-1080 cells, NCI-H1299 cells
Positive IP detected inHepG2 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

19521-1-AP targets NY-ESO-1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cancer/testis antigen 1A
Synonyms Cancer/testis antigen 1, cancer/testis antigen 1A, Cancer/testis antigen 6.1, CT6.1, CTAG, CTAG1, CTAG1A, CTAG1B, ESO1, L antigen family member 2, LAGE 2, LAGE2, LAGE2A, LAGE2B, NY-ESO-1
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 16-25 kDa
GenBank Accession NumberNM_139250
Gene Symbol CTAG1A
Gene ID (NCBI) 246100
RRIDAB_10638144
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78358
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CTAG1A, also named as NY-ESO-1, CTAG, CTAG1, ESO1, LAGE2, LAGE2A, CTAG1B, LAGE2B and CT6.1, belongs to the CTAG family.

实验方案

Product Specific Protocols
WB protocol for NY-ESO-1 antibody 19521-1-APDownload protocol
IHC protocol for NY-ESO-1 antibody 19521-1-APDownload protocol
IF protocol for NY-ESO-1 antibody 19521-1-APDownload protocol
IP protocol for NY-ESO-1 antibody 19521-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancer Immunol Res

CD8+ T cell-Dependent Remodeling of the Tumor Microenvironment Overcomes Chemoresistance

Authors - Liyan Lao
View Article
humanWB

Nat Cancer

The IRENA lncRNA converts chemotherapy-polarized tumor-suppressing macrophages to tumor-promoting phenotypes in breast cancer.

Authors - Jiang Liu
View Article