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验证数据展示

  • WB analysis using 14206-1-AP

    WB analysis using 14206-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14206-1-AP (CSTF2T antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 14206-1-AP

    IHC staining of human prostate cancer using 14206-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14206-1-AP (CSTF2T antibody) at dilution of 1:100 (under 40x lens).

  • IHC staining of human prostate cancer using 14206-1-AP

    IHC staining of human prostate cancer using 14206-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 14206-1-AP (CSTF2T antibody) at dilution of 1:100 (under 10x lens).

  • IP experiment of mouse brain using 14206-1-AP

    IP experiment of mouse brain using 14206-1-AP

    IP result of anti-CSTF2T (IP:14206-1-AP, 4ug; Detection:14206-1-AP 1:1000) with mouse brain tissue lysate 5200ug.

  • IF Staining of HepG2 using 14206-1-AP

    IF Staining of HepG2 using 14206-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 14206-1-AP (CSTF2T antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

CSTF2T Polyclonal antibody

CSTF2T Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14206-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

TauCstF-64, TauCstF 64, KIAA0689, CstF 64T, Cleavage stimulation factor 64 kDa subunit tau variant

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, rat brain tissue, human brain tissue, HeLa cells, HepG2 cells, mouse liver tissue
Positive IP detected inmouse brain tissue, HeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14206-1-AP targets CSTF2T in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5411

Product name: Recombinant human CSTF2T protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 277-616 aa of BC028239

Sequence: PGSLTPGGAMQPQLGMPGVGPVPLERGQVQMSDPRAPIPRGPVTPGGLPPRGLLGDAPNDPRGGTLLSVTGEVEPRGYLGPPHQGPPMHHASGHDTRGPSSHEMRGGPLGDPRLLIGEPRGPMIDQRGLPMDGRGGRDSRAMETRAMETEVLETRVMERRGMETCAMETRGMEARGMDARGLEMRGPVPSSRGPMTGGIQGPGPINIGAGGPPQGPRQVPGISGVGNPGAGMQGTGIQGTGMQGAGIQGGGMQGAGIQGVSIQGGGIQGGGIQGASRQGGSQPSSFSPGQSQVTPQDQEKAALIMQVLQLTADQIAMLPPEQRQSILILKEQIQKSTGAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa, tau variant
别名 TauCstF-64, TauCstF 64, KIAA0689, CstF 64T, Cleavage stimulation factor 64 kDa subunit tau variant
计算分子量 64 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC028239
基因名称 CSTF2T
Gene ID (NCBI) 23283
RRIDAB_2086478
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H0L4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CSTF2T antibody 14206-1-APDownload protocol
IHC protocol for CSTF2T antibody 14206-1-APDownload protocol
IF protocol for CSTF2T antibody 14206-1-APDownload protocol
IP protocol for CSTF2T antibody 14206-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols