验证数据展示
经过测试的应用
Positive WB detected in | mouse heart tissue, human heart tissue, rat heart tissue |
Positive IP detected in | mouse heart tissue |
Positive IHC detected in | human heart tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 3 publications below |
IHC | See 1 publications below |
产品信息
10721-1-AP targets CSRP3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IP, ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | CSRP3 fusion protein Ag1136 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | cysteine and glycine-rich protein 3 (cardiac LIM protein) |
别名 | Cardiac LIM protein, CMD1M, CRP3, Cysteine and glycine-rich protein 3, Cysteine rich protein 3 |
计算分子量 | 21 kDa |
观测分子量 | 21 kDa |
GenBank蛋白编号 | BC005900 |
基因名称 | CSRP3 |
Gene ID (NCBI) | 8048 |
RRID | AB_2292475 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P50461 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CSRP3/MLP (cysteine-rich protein 3/muscle Lim protein), a member of the cysteine-rich protein family, is a muscle-specific LIM-only factor specifically expressed in skeletal muscle. The cytoplasmic CSRP3 serves as a scaffold protein that primarily interacts and co-localizes with different Z-disc components of myofibrils (PMID: 31979369, 17519359).
实验方案
Product Specific Protocols | |
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WB protocol for CSRP3 antibody 10721-1-AP | Download protocol |
IHC protocol for CSRP3 antibody 10721-1-AP | Download protocol |
IP protocol for CSRP3 antibody 10721-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
FASEB J Amplified pathogenic actions of angiotensin II in cysteine-rich LIM-only protein 4-negative mouse hearts. | ||
Hum Mol Genet Misregulation of calcium-handling proteins promotes hyperactivation of calcineurin-NFAT signaling in skeletal muscle of DM1 mice. | ||
J Gene Med CSRP3, p.Arg122*, is Responsible for Hypertrophic Cardiomyopathy in a Chinese Family. | ||
Nat Commun Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis |