CSRP3 Polyclonal antibody

CSRP3 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 10721-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

Cardiac LIM protein, CMD1M, CRP3, Cysteine and glycine-rich protein 3, Cysteine rich protein 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse heart tissue, human heart tissue, rat heart tissue
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman heart tissue, human pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10721-1-AP targets CSRP3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 CSRP3 fusion protein Ag1136 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cysteine and glycine-rich protein 3 (cardiac LIM protein)
别名 Cardiac LIM protein, CMD1M, CRP3, Cysteine and glycine-rich protein 3, Cysteine rich protein 3
计算分子量 21 kDa
观测分子量 21 kDa
GenBank蛋白编号BC005900
基因名称 CSRP3
Gene ID (NCBI) 8048
RRIDAB_2292475
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50461
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CSRP3/MLP (cysteine-rich protein 3/muscle Lim protein), a member of the cysteine-rich protein family, is a muscle-specific LIM-only factor specifically expressed in skeletal muscle. The cytoplasmic CSRP3 serves as a scaffold protein that primarily interacts and co-localizes with different Z-disc components of myofibrils (PMID: 31979369, 17519359).

实验方案

Product Specific Protocols
WB protocol for CSRP3 antibody 10721-1-APDownload protocol
IHC protocol for CSRP3 antibody 10721-1-APDownload protocol
IP protocol for CSRP3 antibody 10721-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

FASEB J

Amplified pathogenic actions of angiotensin II in cysteine-rich LIM-only protein 4-negative mouse hearts.

Authors - Julia Straubinger
mouseWB

Hum Mol Genet

Misregulation of calcium-handling proteins promotes hyperactivation of calcineurin-NFAT signaling in skeletal muscle of DM1 mice.

Authors - Aymeric Ravel-Chapuis
humanIHC

J Gene Med

CSRP3, p.Arg122*, is Responsible for Hypertrophic Cardiomyopathy in a Chinese Family.

Authors - Hao Huang
mouseWB

Nat Commun

Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis

Authors - Yesheng Fu
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