验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HEK-293 cells, HeLa cells, mouse testis tissue, rat testis tissue |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy CSPP1 Ready-To-Use IHC Kit. CSPP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
11931-1-AP targets CSPP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IP, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | CSPP1 fusion protein Ag2521 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | centrosome and spindle pole associated protein 1 |
| 别名 | CSPP, Centrosome and spindle pole-associated protein 1 |
| 计算分子量 | 141 kDa |
| 观测分子量 | 141 kDa |
| GenBank蛋白编号 | BC029445 |
| 基因名称 | CSPP1 |
| Gene ID (NCBI) | 79848 |
| RRID | AB_2087897 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q1MSJ5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CSPP1 interacts with centrosomes and microtubules and plays a role in cell-cycle progression and spindle organization. CSPP1 is required for their common C-terminal domain to interact with Nephrocystin 8 (NPHP8/RPGRIP1L) and to form a ternary complex with NPHP8 and NPHP4, and for ciliogenesis in hTERT-RPE1 cells in vitro and are expressed in ciliated renal, retinal, and respiratory cells in vivo
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CSPP1 antibody 11931-1-AP | Download protocol |
| IF protocol for CSPP1 antibody 11931-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Hum Genet Mutations in CSPP1 Cause Primary Cilia Abnormalities and Joubert Syndrome with or without Jeune Asphyxiating Thoracic Dystrophy. | ||
Cell Rep A CEP104-CSPP1 Complex Is Required for Formation of Primary Cilia Competent in Hedgehog Signaling.
| ||
EMBO Rep CK1-mediated phosphorylation of FAM110A promotes its interaction with mitotic spindle and controls chromosomal alignment.
| ||
PLoS Biol The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization. | ||
Br J Cancer Nuclear CSPP1 expression defined subtypes of basal-like breast cancer.
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