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验证数据展示

  • WB analysis using 16384-1-AP (same clone as 16384-1-PBS)

    WB analysis using 16384-1-AP (same clone as 16384-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 16384-1-AP (same clone as 16384-1-PBS)

    IHC staining of human liver using 16384-1-AP (same clone as 16384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16384-1-AP (CSNK1G1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CSNK1G1 antibody included
  • WB analysis of Jurkat using 16384-1-AP (same clone as 16384-1-PBS)

    WB analysis of Jurkat using 16384-1-AP (same clone as 16384-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 16384-1-AP (same clone as 16384-1-PBS)

    IHC staining of human liver using 16384-1-AP (same clone as 16384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16384-1-AP (CSNK1G1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CSNK1G1 antibody included
  • IP experiment of Jurkat using 16384-1-AP (same clone as 16384-1-PBS)

    IP experiment of Jurkat using 16384-1-AP (same clone as 16384-1-PBS)

    IP result of anti-CSNK1G1 (IP:16384-1-AP, 4ug; Detection:16384-1-AP 1:300) with Jurkat cells lysate 2800ug. This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 16384-1-AP (same clone as 16384-1-PBS)

    IHC staining of mouse testis using 16384-1-AP (same clone as 16384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16384-1-AP (CSNK1G1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CSNK1G1 antibody included
  • IHC staining of human pancreas cancer using 16384-1-AP (same clone as 16384-1-PBS)

    IHC staining of human pancreas cancer using 16384-1-AP (same clone as 16384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16384-1-AP (CSNK1G1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CSNK1G1 antibody included
  • IHC staining of human tonsillitis using 16384-1-AP (same clone as 16384-1-PBS)

    IHC staining of human tonsillitis using 16384-1-AP (same clone as 16384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 16384-1-AP (CSNK1G1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CSNK1G1 antibody included
  • IHC staining of rat lung using 16384-1-AP (same clone as 16384-1-PBS)

    IHC staining of rat lung using 16384-1-AP (same clone as 16384-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 16384-1-AP (CSNK1G1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16384-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CSNK1G1 antibody included

CSNK1G1 Polyclonal antibody, PBS Only

CSNK1G1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 16384-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, monkey

应用

WB, IHC, IP, Indirect ELISA

别名

Casein kinase I isoform gamma-1, CKI gamma 1, CKI-gamma 1, CSNK, CSNK1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy CSNK1G1 Ready-To-Use IHC Kit. CSNK1G1 primary antibody included.

产品信息

16384-1-PBS targets CSNK1G1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, monkey
免疫原

CatNo: Ag9395

Product name: Recombinant human CSNK1G1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 22-398 aa of BC017236

Sequence: VGKKIGCGNFGELRLGKNLYTNEYVAIKLEPIKSRAPQLHLEYRFYKQLGSAGEGLPQVYYFGPCGKYNAMVLELLGPSLEDLFDLCDRTFTLKTVLMIAIQLLSRMEYVHSKNLIYRDVKPENFLIGRQGNKKEHVIHIIDFGLAKEYIDPETKKHIPYREHKSLTGTARYMSINTHLGKEQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERYQKIGDTKRNTPIEALCENFPEEMATYLRYVRRLDFFEKPDYEYLRTLFTDLFEKKGYTFDYAYDWVGRPIPTPVGSVHVDSGASAITRESHTHRDRPSQQQPLRNQVVSSTNGELNVDDPTGAHSNAPITAHAEVEVVEEAKCCCFFKRKRKKTAQRHK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 casein kinase 1, gamma 1
别名 Casein kinase I isoform gamma-1, CKI gamma 1, CKI-gamma 1, CSNK, CSNK1
计算分子量 422 aa, 49 kDa
观测分子量 49 kDa
GenBank蛋白编号BC017236
基因名称 CSNK1G1
Gene ID (NCBI) 53944
RRIDAB_2087300
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HCP0
储存缓冲液PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The casein kinase 1 (CK1) is a family of Ser/Thr protein kinases that functions as regulator in signal transduction of eukaryotic cells. CSNK1G1(Casein kinase I isoform gamma-1) plays an important role in activating the ATR/Chk1 pathway by phosphorylating Claspin, which was identified as a Chk1-activating protein. Recently, it has been found that CSNK1G1 can phosphorylate NF-kB subunit p65 at Ser536, which promotes its ubiquitin-mediated degredation by E3 ligases COMMD1 and CUL2.(PMID:24442433). This protein has 2 isoforms produced by alternative splicing with the molecular weight of 49 kDa and 45 kDa.

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