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验证数据展示

  • WB analysis using 16384-1-AP

    WB analysis using 16384-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16384-1-AP

    IHC staining of human liver using 16384-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16384-1-AP (CSNK1G1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CSNK1G1 antibody included
  • WB analysis of Jurkat using 16384-1-AP

    WB analysis of Jurkat using 16384-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16384-1-AP

    IHC staining of human liver using 16384-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16384-1-AP (CSNK1G1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CSNK1G1 antibody included
  • IP experiment of Jurkat using 16384-1-AP

    IP experiment of Jurkat using 16384-1-AP

    IP result of anti-CSNK1G1 (IP:16384-1-AP, 4ug; Detection:16384-1-AP 1:300) with Jurkat cells lysate 2800ug.

CSNK1G1 Polyclonal antibody

CSNK1G1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 16384-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human, monkey

应用

WB, IP, IHC, ELISA

别名

casein kinase 1, gamma 1, CKI gamma 1, CSNK1G1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, COS-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CSNK1G1 Ready-To-Use IHC Kit. CSNK1G1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16384-1-AP targets CSNK1G1 in WB, IP, IHC, ELISA applications and shows reactivity with human, monkey samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IP
经测试反应性 human, monkey
文献引用反应性human
免疫原

CatNo: Ag9395

Product name: Recombinant human CSNK1G1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 22-398 aa of BC017236

Sequence: VGKKIGCGNFGELRLGKNLYTNEYVAIKLEPIKSRAPQLHLEYRFYKQLGSAGEGLPQVYYFGPCGKYNAMVLELLGPSLEDLFDLCDRTFTLKTVLMIAIQLLSRMEYVHSKNLIYRDVKPENFLIGRQGNKKEHVIHIIDFGLAKEYIDPETKKHIPYREHKSLTGTARYMSINTHLGKEQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERYQKIGDTKRNTPIEALCENFPEEMATYLRYVRRLDFFEKPDYEYLRTLFTDLFEKKGYTFDYAYDWVGRPIPTPVGSVHVDSGASAITRESHTHRDRPSQQQPLRNQVVSSTNGELNVDDPTGAHSNAPITAHAEVEVVEEAKCCCFFKRKRKKTAQRHK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 casein kinase 1, gamma 1
别名 casein kinase 1, gamma 1, CKI gamma 1, CSNK1G1
计算分子量 422 aa, 49 kDa
观测分子量 49 kDa
GenBank蛋白编号BC017236
基因名称 CSNK1G1
Gene ID (NCBI) 53944
RRIDAB_2087300
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HCP0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The casein kinase 1 (CK1) is a family of Ser/Thr protein kinases that functions as regulator in signal transduction of eukaryotic cells. CSNK1G1(Casein kinase I isoform gamma-1) plays an important role in activating the ATR/Chk1 pathway by phosphorylating Claspin, which was identified as a Chk1-activating protein. Recently, it has been found that CSNK1G1 can phosphorylate NF-kB subunit p65 at Ser536, which promotes its ubiquitin-mediated degredation by E3 ligases COMMD1 and CUL2.(PMID:24442433). This protein has 2 isoforms produced by alternative splicing with the molecular weight of 49 kDa and 45 kDa.

实验方案

Product Specific Protocols
WB protocol for CSNK1G1 antibody 16384-1-APDownload protocol
IHC protocol for CSNK1G1 antibody 16384-1-APDownload protocol
IP protocol for CSNK1G1 antibody 16384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

J Immunol

Casein Kinase 1γ1 Inhibits the RIG-I/TLR Signaling Pathway through Phosphorylating p65 and Promoting Its Degradation.

Authors - Yetao Wang
View Article