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CSNK1G1 Polyclonal antibody

CSNK1G1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, monkey

Applications

IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 16384-1-AP

Print datasheet

Synonyms

casein kinase 1, gamma 1, CKI gamma 1, CSNK1G1

验证数据展示

  • WB analysis using 16384-1-AP

    WB analysis using 16384-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16384-1-AP

    IHC staining of human liver using 16384-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16384-1-AP (CSNK1G1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Jurkat using 16384-1-AP

    WB analysis of Jurkat using 16384-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 16384-1-AP (CSNK1G1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 16384-1-AP

    IHC staining of human liver using 16384-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 16384-1-AP (CSNK1G1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of Jurkat using 16384-1-AP

    IP experiment of Jurkat using 16384-1-AP

    IP result of anti-CSNK1G1 (IP:16384-1-AP, 4ug; Detection:16384-1-AP 1:300) with Jurkat cells lysate 2800ug.

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells, COS-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16384-1-AP targets CSNK1G1 in WB, IP, IHC, ELISA applications and shows reactivity with human, monkey samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB, IP
Tested Reactivity human, monkey
Cited Reactivityhuman
Immunogen CSNK1G1 fusion protein Ag9395 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name casein kinase 1, gamma 1
Synonyms casein kinase 1, gamma 1, CKI gamma 1, CSNK1G1
Calculated Molecular Weight 422 aa, 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC017236
Gene Symbol CSNK1G1
Gene ID (NCBI) 53944
RRIDAB_2087300
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HCP0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The casein kinase 1 (CK1) is a family of Ser/Thr protein kinases that functions as regulator in signal transduction of eukaryotic cells. CSNK1G1(Casein kinase I isoform gamma-1) plays an important role in activating the ATR/Chk1 pathway by phosphorylating Claspin, which was identified as a Chk1-activating protein. Recently, it has been found that CSNK1G1 can phosphorylate NF-kB subunit p65 at Ser536, which promotes its ubiquitin-mediated degredation by E3 ligases COMMD1 and CUL2.(PMID:24442433). This protein has 2 isoforms produced by alternative splicing with the molecular weight of 49 kDa and 45 kDa.

实验方案

Product Specific Protocols
WB protocol for CSNK1G1 antibody 16384-1-APDownload protocol
IHC protocol for CSNK1G1 antibody 16384-1-APDownload protocol
IP protocol for CSNK1G1 antibody 16384-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IP

J Immunol

Casein Kinase 1γ1 Inhibits the RIG-I/TLR Signaling Pathway through Phosphorylating p65 and Promoting Its Degradation.

Authors - Yetao Wang
View Article