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验证数据展示

  • WB analysis using 31573-1-AP

    WB analysis using 31573-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31573-1-AP (CSMD2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 31573-1-AP

    IHC staining of mouse brain using 31573-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31573-1-AP (CSMD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 31573-1-AP

    IHC staining of mouse brain using 31573-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31573-1-AP (CSMD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CSMD2 Polyclonal antibody

CSMD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31573-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31573-1-AP targets CSMD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag36106

Product name: Recombinant human CSMD2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 3246-3408 aa of NM_001281956

Sequence: LEVRPSGRPINTAREPPLTQALIPGDVFAKNSLWKGAYEYQGKKQPAMLRVTGFQVANSKVNATMIDHSGVELHLAGTYKKEDFHLLLQVYQITGPVEIFMNKFKDDHWALDGHVSSESSGATFIYQGSVKGQGFGQFGFQRLDLRLLESDPESIGRHFASNS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 CUB and Sushi multiple domains 2
别名
计算分子量380kDa
观测分子量380 kDa
GenBank蛋白编号NM_001281956
基因名称 CSMD2
Gene ID (NCBI) 114784
RRIDAB_3670040
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ7Z408
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CUB and sushi multiple domains 2 (CSMD2), located on the short arm of human chromosome 1 (1p35.1), encodes a single-pass transmembrane protein with a large extracellular domain consisting of CUB and Sushi domain repeats. CSMD2 is highly expressed in the developing and mature brain and has been linked to schizophrenia (PMID:31068362). The full-length molecular weight of CSMD2 protein is 380 kDa.

实验方案

Product Specific Protocols
IHC protocol for CSMD2 antibody 31573-1-APDownload protocol
WB protocol for CSMD2 antibody 31573-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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