验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, rat kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33679-1-AP targets CRYL1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag38269 Product name: Recombinant human CRYL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-319 aa of NM_015974.2 Sequence: MASSAAGCVVIVGSGVIGRSWAMLFASGGFQVKLYDIEQQQIRNALENIRKEMKLLEQAGSLKGSLSVEEQLSLISGCPNIQEAVEGAMHIQECVPEDLELKKKIFAQLDSIIDDRVILSSSTSCLMPSKLFAGLVHVKQCIVAHPVNPPYYIPLVELVPHPETAPTTVDRTHALMKKIGQCPMRVQKEVAGFVLNRLQYAIISEAWRLVEEGIVSPSDLDLVMSEGLGMRYAFIGPLETMHLNAEGMLSYCDRYSEGIKHVLQTFGPIPEFSRATAEKVNQDMCMKVPDDPEHLAARRQWRDECLMRLAKLKSQVQPQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | crystallin, lambda 1 |
| 别名 | CRY, EC:1.1.1.45, Gul3DH, Lambda-crystallin homolog, L-gulonate 3-dehydrogenase |
| 计算分子量 | 35kDa，319aa |
| 观测分子量 | 35 kDa |
| GenBank蛋白编号 | NM_015974.2 |
| 基因名称 | CRYL1 |
| Gene ID (NCBI) | 51084 |
| RRID | AB_3743046 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q9Y2S2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRYL1 (Crystallin Lambda 1) is a ubiquitously-expressed, dual-function protein that was first isolated from rabbit lens tissue. Human CRYL1 is encoded at chromosomal locus 13q12.11, contains two HCDH (3-hydroxyacyl-CoA dehydrogenase) domains, and is most abundant in liver and kidney, with only trace expression in peripheral-blood leukocytes and thymus. Further research showed that CRYL1 was expressed at lower levels in HCC tumors than in adjacent tissues, and this reduced expression was associated with advanced tumor staging, larger tumors, and decreased patient disease-free survival.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CRYL1 antibody 33679-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
