验证数据展示
经过测试的应用
| Positive WB detected in | mouse eye tissue, rat eye tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32233-1-AP targets CRYGD in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag37473 Product name: Recombinant human CRYGD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-120 aa of NM_006891.3 Sequence: MGKITLYEDRGFQGRHYECSSDHPNLQPYLSRCNSARVDSGCWMLYEQPNYSGLQYFLRRGDYADHQQWMGLSDSVRSCRLIPHSGSHRIRLYEREDYRGQMIEFTEDCSCLQDRFRFNE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | crystallin, gamma D |
| 别名 | CRYG4, Gamma-crystallin 4, Gamma-crystallin D, Gamma-D-crystallin |
| 计算分子量 | 21kDa，174aa |
| 观测分子量 | 18 kDa |
| GenBank蛋白编号 | NM_006891.3 |
| 基因名称 | CRYGD |
| Gene ID (NCBI) | 1421 |
| RRID | AB_3742550 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | P07320 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRYGD (Crystallin Gamma D) is a structural protein predominantly expressed in the lens of the eye. It plays a critical role in maintaining lens transparency and refractive index. Mutations in the CRYGD gene are associated with inherited forms of congenital cataracts, making it a genetic target for studying lens development and cataract pathogenesis. It is not a conventional therapeutic drug target but serves as a key marker in ocular disease research.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CRYGD antibody 32233-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
