• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of C6 using 60273-1-Ig

    WB analysis of C6 using 60273-1-Ig

    C6 cells were subjected to SDS PAGE followed by western blot with 60273-1-Ig (CRYBB1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of rat eye using 60273-1-Ig

    WB analysis of rat eye using 60273-1-Ig

    rat eye tissue were subjected to SDS PAGE followed by western blot with 60273-1-Ig (CRYBB1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of hTERT-RPE1 using 60273-1-Ig

    IF Staining of hTERT-RPE1 using 60273-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CRYBB1 antibody (60273-1-Ig, Clone: 6F12B12 ) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

CRYBB1 Monoclonal antibody

CRYBB1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 60273-1-Ig

产品说明书

CloneNo. 6F12B12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

6F12B12, Beta B1 crystallin, Beta crystallin B1, Beta-B1 crystallin, Beta-crystallin B1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inrat eye tissue, C6 cells, mouse eye tissue
Positive IF/ICC detected inhTERT-RPE1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60273-1-Ig targets CRYBB1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CRYBB1 fusion protein Ag4722 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 crystallin, beta B1
别名 6F12B12, Beta B1 crystallin, Beta crystallin B1, Beta-B1 crystallin, Beta-crystallin B1
计算分子量 252 aa, 28 kDa
观测分子量 30 kDa
GenBank蛋白编号BC036790
基因名称 CRYBB1
Gene ID (NCBI) 1414
RRIDAB_2881393
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP53674
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mutation in CRYBB1 is associated with congenital Cataract-Microcornea Syndrome.

实验方案

Product Specific Protocols
WB protocol for CRYBB1 antibody 60273-1-IgDownload protocol
IF protocol for CRYBB1 antibody 60273-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols