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验证数据展示

  • WB analysis using 86690-1-RR

    WB analysis using 86690-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86690-1-RR (CROP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 86690-1-RR

    WB analysis using 86690-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86690-1-RR (CROP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 86690-1-RR

    IF Staining of HepG2 using 86690-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CROP antibody (86690-1-RR, Clone: 251672C12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 86690-1-RR

    Affinity and Kinetic Characterization of 86690-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86690-1-RR against Human CROP were performed. The affinity constant is 3.07 nM.

CROP Recombinant monoclonal antibody

CROP Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 86690-1-RR

产品说明书

CloneNo. 251672C12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

cAMP regulatory element-associated protein 1, Cisplatin resistance-associated-overexpressed protein, CRE associated protein 1, CREAP 1, CREAP1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inJurkat cells, RT-4 cells, HepG2 cells, mouse brain tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86690-1-RR targets CROP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag5961

Product name: Recombinant human CROP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-79 aa of BC056409

Sequence: MISAAQLLDELMGRDRNLAPDEKRSNVRWDHESVCKYYLCGFCPAELFTNTRSDLDVFGRGDNISDVSKFLEDDKWMEE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cisplatin resistance-associated overexpressed protein
别名 cAMP regulatory element-associated protein 1, Cisplatin resistance-associated-overexpressed protein, CRE associated protein 1, CREAP 1, CREAP1
计算分子量 51 kDa
观测分子量 55-58 kDa
GenBank蛋白编号BC056409
基因名称 CROP
Gene ID (NCBI) 51747
RRIDAB_3745055
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO95232
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CROP, a putative SR protein, contain cystine/histidine motifs and leucine zipper-like repeats in the N-terminal half and consists mostly of charged and polar amino acids in the C-terminal half. CROP has a high expression in heart, brain, pancreas, thymus, and weak in lung, live and kidney. As the human homologue of yeast Luc7p, CROP is supported to be participated in 5'-splice site recognize and is essential for vegetative growth. This antibody raised against part of N-terminus of CROP of human origin, and can recognize a ~58kDa band of CROP.

实验方案

Product Specific Protocols
IF protocol for CROP antibody 86690-1-RRDownload protocol
WB protocol for CROP antibody 86690-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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