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验证数据展示

  • WB analysis of mouse heart using 14801-1-AP

    WB analysis of mouse heart using 14801-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 14801-1-AP (CRIP2 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 14801-1-AP

    IF Staining of HeLa using 14801-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CRIP2 antibody (14801-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Featured Product
  • KD/KO验证

CRIP2 Polyclonal antibody

CRIP2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 14801-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IF/ICC, ELISA and More (2)

别名

CRIP, CRIP 2, CRP 2, CRP2, CRP-2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inmouse heart tissue, HeLa cells, MCF-7 cells, mouse brain tissue, mouse lung tissue
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14801-1-AP targets CRIP2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, rat, zebrafish
免疫原 CRIP2 fusion protein Ag6515 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cysteine-rich protein 2
别名 CRIP, CRIP 2, CRP 2, CRP2, CRP-2
计算分子量 22 kDa
观测分子量 23-29 kDa
GenBank蛋白编号BC000434
基因名称 CRIP2
Gene ID (NCBI) 1397
RRIDAB_2245316
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP52943
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRIP2, also known as CRP2 and ESP1, belongs to the cysteine-rich intestinal protein family (CRIP family). The CRIP family is a subfamily of the highly conserved Lin-1, Isl1, Mec3/double zinc finger protein family that exhibits diverse biological functions. CRIP2 is a cardiac vascular marker, and its expression is detected in cardiac endothelial cells during development and in the adult heart(PMID: 40118853).

实验方案

Product Specific Protocols
WB protocol for CRIP2 antibody 14801-1-APDownload protocol
IF protocol for CRIP2 antibody 14801-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,IP,IF

Basic Res Cardiol

Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart.

Authors - Alzbeta Hulikova
View Article
zebrafish,humanWB,IF

Cell Mol Life Sci

Crip2 affects vascular development by fine-tuning endothelial cell aggregation and proliferation

Authors - Shuaiqi Yang
  • KD Validated
View Article