验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, HeLa cells, MCF-7 cells, mouse brain tissue, mouse lung tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
14801-1-AP targets CRIP2 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat, zebrafish |
| 免疫原 |
CatNo: Ag6515 Product name: Recombinant human CRIP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-208 aa of BC000434 Sequence: MASKCPKCDKTVYFAEKVSSLGKDWHKFCLKCERCSKTLTPGGHAEHDGKPFCHKPCYATLFGPKGVNIGGAGSYIYEKPLAEGPQVTGPIEVPAARAEERKASGPPKGPSRASSVTTFTGEPNTCPRCSKKVYFAEKVTSLGKDWHRPCLRCERCGKTLTPGGHAEHDGQPYCHKPCYGILFGPKGVNTGAVGSYIYDRDPEGKVQP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cysteine-rich protein 2 |
| 别名 | CRIP, CRIP 2, CRP 2, CRP2, CRP-2 |
| 计算分子量 | 22 kDa |
| 观测分子量 | 23-29 kDa |
| GenBank蛋白编号 | BC000434 |
| 基因名称 | CRIP2 |
| Gene ID (NCBI) | 1397 |
| RRID | AB_2245316 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P52943 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRIP2, also known as CRP2 and ESP1, belongs to the cysteine-rich intestinal protein family (CRIP family). The CRIP family is a subfamily of the highly conserved Lin-1, Isl1, Mec3/double zinc finger protein family that exhibits diverse biological functions. CRIP2 is a cardiac vascular marker, and its expression is detected in cardiac endothelial cells during development and in the adult heart(PMID: 40118853).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CRIP2 antibody 14801-1-AP | Download protocol |
| WB protocol for CRIP2 antibody 14801-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Basic Res Cardiol Alkaline nucleoplasm facilitates contractile gene expression in the mammalian heart. | ||
Cell Mol Life Sci Crip2 affects vascular development by fine-tuning endothelial cell aggregation and proliferation
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