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验证数据展示

  • WB analysis using 12439-1-AP (same clone as 12439-1-PBS)

    WB analysis using 12439-1-AP (same clone as 12439-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12439-1-AP (CREST antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 12439-1-AP (same clone as 12439-1-PBS)

    IP experiment of HeLa using 12439-1-AP (same clone as 12439-1-PBS)

    IP result of anti-CREST (IP:12439-1-AP, 4ug; Detection:12439-1-AP 1:300) with HeLa cells lysate 3000 ug. This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 12439-1-AP (same clone as 12439-1-PBS)

    IHC staining of human breast cancer using 12439-1-AP (same clone as 12439-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 12439-1-AP (CREST antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 12439-1-AP (same clone as 12439-1-PBS)

    IF Staining of mouse testis using 12439-1-AP (same clone as 12439-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using 12439-1-AP (CREST antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 12439-1-AP (same clone as 12439-1-PBS)

    IF Staining of HeLa using 12439-1-AP (same clone as 12439-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CREST antibody (12439-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 12439-1-AP (same clone as 12439-1-PBS)

    WB analysis of HeLa using 12439-1-AP (same clone as 12439-1-PBS)

    WB result of CREST antibody (12439-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CREST transfected HeLa cells. This data was developed using the same antibody clone with 12439-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

CREST Polyclonal antibody, PBS Only

CREST Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA
Cat No. 12439-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, IP, ELISA

别名

SS18L1, Calcium-responsive transactivator, KIAA0693, LP2261, SS18 like protein 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

12439-1-PBS targets CREST in WB, IHC, IF/ICC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3119

Product name: Recombinant human CREST protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 47-396 aa of BC034494

Sequence: CTQYQQILHRNLVYLATIADSNQNMQSLLPAPPTQNMNLGPGALTQSGSSQGLHSQGSLSDAISTGLPPSSLLQGQIGNGPSHVSMQQTAPNTLPTTSMSISGPGYSHAGPASQGVPMQGQGTIGNYVSRTNINMQSNPVSMIQQQAATSHYSSAQGGSQHYQGQSSIAMMGQGSQGSSMMGQRPMAPYRPSQQGSSQQYLGQEEYYGEQYSHSQGAAEPMGQQYYPDGHGDYAYQQSSYTEQSYDRSFEESTQHYYEGGNSQYSQQQAGYQQGAAQQQTYSQQQYPSQQSYPGQQQGYGSAQGAPSQYPGYQQGQGQQYGSYRAPQTAPSAQQQRPYGYEQGQYGNYQQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 synovial sarcoma translocation gene on chromosome 18-like 1
别名 SS18L1, Calcium-responsive transactivator, KIAA0693, LP2261, SS18 like protein 1
计算分子量 396 aa, 43 kDa
观测分子量 55 kDa
GenBank蛋白编号BC034494
基因名称 CREST
Gene ID (NCBI) 26039
RRIDAB_2302517
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75177
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CREST, also named as SS18-like 1(SS18L1) is a transcriptional activator that is required for calcium-dependent dendritic growth and branching in cortical neurons. It's also a nuclear protein interacts with CREB-binding protein and expressed in the developing brain. It helps regulate neuronal morphogenesis in calcuim-dependent manner. The N-terminal domain of SS18L1 is required for suppressing transactivation in the basal state, while the C-terminal domain is required for calcium-induced transactivation. It's part of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. This complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves a release of HDAC1 and recruitment of CREBBP. The calculated molecular weight of CREST is about 43 kDa, but the modified of CREST protein is 55 kDa (PMID: 25888396). CREST exists some isoforms with calulated MV 43, 40, 33 and 29 kDa.

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