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验证数据展示

  • IHC staining of mouse brain using 28236-1-AP

    IHC staining of mouse brain using 28236-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28236-1-AP (CREG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 28236-1-AP

    IHC staining of mouse brain using 28236-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28236-1-AP (CREG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of NIH/3T3 using 28236-1-AP

    IF Staining of NIH/3T3 using 28236-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CREG2 antibody (28236-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CREG2 Polyclonal antibody

CREG2 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 28236-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, ELISA

别名

Protein CREG2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28236-1-AP targets CREG2 in IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag28242

Product name: Recombinant human CREG2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 42-215 aa of BC032949

Sequence: VTNEVDEELDSASTEEAMPALLEDSGSIWQQSFPASAHKEDAHLRPRAGAARARQPPAPPGMFSYRREGGQTASAPPGPRLRAATARSLAHASVWGCLATVSTHKKIQGLPFGNCLPVSDGPFNNSTGIPFFYMTAKDPVVADLMKNPMASLMLPESEGEFCRKNIVDPEDPRC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cellular repressor of E1A-stimulated genes 2
别名 Protein CREG2
计算分子量 32 kDa
GenBank蛋白编号BC032949
基因名称 CREG2
Gene ID (NCBI) 200407
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IUH2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The cellular repressor of E1A-stimulated genes (CREG) is a secreted glycoprotein that promotes the differentiation of pluripotent stem cells. CREG2 is a promising biological indicator because it plays an important role in increasing the migratory and proliferative capacity of lung adenocarcinoma (LUAD) cells (PMID: 40676398).

实验方案

Product Specific Protocols
IF protocol for CREG2 antibody 28236-1-APDownload protocol
IHC protocol for CREG2 antibody 28236-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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