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CREB3L4 Polyclonal antibody

CREB3L4 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 13630-1-AP

Print datasheet

Synonyms

AIBZIP, ATCE1, Attaching to CRE like 1, CREB 4, CREB3, CREB3L4, CREB4, hJAL, JAL, Tisp40

验证数据展示

  • WB analysis using 13630-1-AP

    WB analysis using 13630-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13630-1-AP (CREB3L4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 13630-1-AP

    IF Staining of MCF-7 using 13630-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 13630-1-AP (CREB3L4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, HL-60 cells
Positive IF detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CREB3L4 Ready-To-Use IHC Kit. CREB3L4 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13630-1-AP targets CREB3L4 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity human
Immunogen CREB3L4 fusion protein Ag4548 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cAMP responsive element binding protein 3-like 4
Synonyms AIBZIP, ATCE1, Attaching to CRE like 1, CREB 4, CREB3, CREB3L4, CREB4, hJAL, JAL, Tisp40
Calculated Molecular Weight 395 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC038962
Gene Symbol CREB3L4
Gene ID (NCBI) 148327
RRIDAB_2276550
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TEY5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CREB3L4 (cAMP responsive element binding protein 3-like 4), also named as AIBZIP, CREB4 and ATCE1, is a member of the CREB/ATF transcription factor family. CREB3L4 may play a role in the unfolded protein response, characterized by their regulation of gene expression through the cAMP-responsive element(PMID: 22829733). CREB3L4 is highly expressed in multiple human cancers, such as human breast carcinoma, prostate cancer(PMID: 32026099, PMID: 28338058). The molecular weight of CREB3L4 is 43 kDa and 55 kDa precursor was reported. Due to glycosylation, CREB3L4 can be observed with molecular weights of 35 and 41 kDa (PMID: 25412305, 15938716).

实验方案

Product Specific Protocols
WB protocol for CREB3L4 antibody 13630-1-APDownload protocol
IF protocol for CREB3L4 antibody 13630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols