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验证数据展示

  • WB analysis using 13630-1-AP

    WB analysis using 13630-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 13630-1-AP (CREB3L4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 13630-1-AP

    IF Staining of MCF-7 using 13630-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed MCF-7 cells using 13630-1-AP (CREB3L4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

CREB3L4 Polyclonal antibody

CREB3L4 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 13630-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA and More (1)

别名

cAMP-responsive element-binding protein 4, cAMP-responsive element-binding protein 3-like protein 4, Attaching to CRE-like 1, ATCE1, Androgen-induced basic leucine zipper protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HL-60 cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CREB3L4 Ready-To-Use IHC Kit. CREB3L4 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13630-1-AP targets CREB3L4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag4548

Product name: Recombinant human CREB3L4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-171 aa of BC038962

Sequence: MDLGIPDLLDAWLEPPEDIFSTGSVLELGLHCPPPEVPVTRLQEQGLQGWKSGGDRGCGLQESEPEDFLKLFIDPNEVYCSEASPGSDSGISEDPCHPDSPPAPRATSSPMLYEVVYEAGALERMQGETGPNVGLISIQLDQWSPAFMVPDSCMVSELPFDAHAHILPRAG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cAMP responsive element binding protein 3-like 4
别名 cAMP-responsive element-binding protein 4, cAMP-responsive element-binding protein 3-like protein 4, Attaching to CRE-like 1, ATCE1, Androgen-induced basic leucine zipper protein
计算分子量 395 aa, 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC038962
基因名称 CREB3L4
Gene ID (NCBI) 148327
RRIDAB_2276550
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TEY5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CREB3L4 (cAMP responsive element binding protein 3-like 4), also named as AIBZIP, CREB4 and ATCE1, is a member of the CREB/ATF transcription factor family. CREB3L4 may play a role in the unfolded protein response, characterized by their regulation of gene expression through the cAMP-responsive element(PMID: 22829733). CREB3L4 is highly expressed in multiple human cancers, such as human breast carcinoma, prostate cancer(PMID: 32026099, PMID: 28338058). The molecular weight of CREB3L4 is 43 kDa and 55 kDa precursor was reported. Due to glycosylation, CREB3L4 can be observed with molecular weights of 35 and 41 kDa (PMID: 25412305, 15938716).

实验方案

Product Specific Protocols
WB protocol for CREB3L4 antibody 13630-1-APDownload protocol
IF protocol for CREB3L4 antibody 13630-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

iScience

CREB3L4 promotes hepatocellular carcinoma progression and decreases sorafenib chemosensitivity by promoting RHEB-mTORC1 signaling pathway

Authors - Zhengchen Jiang
  • KD Validated
View Article