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验证数据展示

  • IHC staining of human pancreas using 12315-1-AP

    IHC staining of human pancreas using 12315-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12315-1-AP (CRB3 Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human pancreas using 12315-1-AP

    IHC staining of human pancreas using 12315-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 12315-1-AP (CRB3 Antibody) at dilution of 1:200 (under 40x lens).

CRB3 Polyclonal antibody

CRB3 Polyclonal Antibody for IHC, ELISA
Cat No. 12315-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

CRB3, crumbs homolog 3 (Drosophila), Crumbs protein homolog 3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inhuman pancreas tissue, human skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12315-1-AP targets CRB3 in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原 CRB3 fusion protein Ag2968 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 crumbs homolog 3 (Drosophila)
别名 CRB3, crumbs homolog 3 (Drosophila), Crumbs protein homolog 3
计算分子量 123 aa, 13 kDa
GenBank蛋白编号BC018409
基因名称 CRB3
Gene ID (NCBI) 92359
RRIDAB_2877844
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BUF7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Crumbs is an apical transmembrane protein crucial for epithelial morphogenesis in Drosophila melanogaster embryos. Three isoforms of mammalian Crumbs have been identified, and two have been characterized. Crumbs1 (CRB1) is expressed primarily in the central nervous system, whereas Crumbs3 (CRB3) is mainly expressed in epithelial tissues and skeletal muscles. CRB3 is an N-glycosylated, small transmembrane protein. CRB3 has a conserved cytoplasmic domain containing the two motives GTY and ERLI found in Crumbs, but exhibits a very short extracellular domain without the EGF- and laminin A-like G repeats present in the other Crumbs. Through its cytoplasmic domain and its interactors (e.g., Pals1 and Par6), CRB3 plays a role in apical membrane morphogenesis and tight junction regulation. (PMID: 14718572; 12527193; 15976445)

实验方案

Product Specific Protocols
IHC protocol for CRB3 antibody 12315-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols