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CRB1 Polyclonal antibody

CRB1 Polyclonal Antibody for IHC,IF/ICC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, human

Applications

IHC,IF/ICC,ELISA

Conjugate

Unconjugated

Cat No : 26598-1-AP

Print datasheet

Synonyms

CRB1, Crumbs homolog 1, crumbs homolog 1 (Drosophila), LCA8, RP12

验证数据展示

  • IHC staining of mouse eye using 26598-1-AP

    IHC staining of mouse eye using 26598-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 26598-1-AP

    IF Staining of SH-SY5Y using 26598-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CRB1 antibody (26598-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive IHC detected inmouse eye tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26598-1-AP targets CRB1 in IF, IHC, ELISA applications and shows reactivity with mouse, human samples.

Tested Applications IHC,IF/ICC,ELISA
Tested Reactivity mouse, human
Immunogen CRB1 fusion protein Ag24512 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name crumbs homolog 1 (Drosophila)
Synonyms CRB1, Crumbs homolog 1, crumbs homolog 1 (Drosophila), LCA8, RP12
Calculated Molecular Weight 1406 aa, 154 kDa
Observed Molecular Weight150 kDa
GenBank Accession NumberBC136271
Gene Symbol CRB1
Gene ID (NCBI) 23418
RRIDAB_2918104
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP82279
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRB1, also known as protein crumbs homolog 1, is homologous to Drosophila Crumbs, a protein that is essential for establishing and maintaining apico-basal polarity in epithelia derived from the ectoderm (PMID: 2344615). CRB1 protein is a single transmembrane protein with a large extracellular part, containing three laminin A G domains and 19 epidermal growth-factor-like domains (PMID: 15494026, 18407265, 11734541). The expression of CRB1 is restricted to the retina and brain (PMID: 10508521). Mutations in CRB1 gene cause severe retinal dystrophies, ranging from retinitis pigmentosa (RP) to Leber congenital amaurosis (LCA) (PMID: 11734541).

实验方案

Product Specific Protocols
IHC protocol for CRB1 antibody 26598-1-APDownload protocol
IF protocol for CRB1 antibody 26598-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols