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验证数据展示

  • IHC staining of mouse eye using 26598-1-AP

    IHC staining of mouse eye using 26598-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 26598-1-AP (CRB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse eye using 26598-1-AP

    IF Staining of mouse eye using 26598-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using CRB1 antibody (26598-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of SH-SY5Y using 26598-1-AP

    IF Staining of SH-SY5Y using 26598-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using CRB1 antibody (26598-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

CRB1 Polyclonal antibody

CRB1 Polyclonal Antibody for IHC, IF/ICC, IF-P, ELISA
Cat No. 26598-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, IF-P, ELISA

别名

Crumbs homolog 1, crumbs homolog 1 (Drosophila), LCA8, Protein crumbs homolog 1, RP12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IHC detected inmouse eye tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26598-1-AP targets CRB1 in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 CRB1 fusion protein Ag24512 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 crumbs homolog 1 (Drosophila)
别名 Crumbs homolog 1, crumbs homolog 1 (Drosophila), LCA8, Protein crumbs homolog 1, RP12
计算分子量 1406 aa, 154 kDa
观测分子量150 kDa
GenBank蛋白编号BC136271
基因名称 CRB1
Gene ID (NCBI) 23418
RRIDAB_2918104
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP82279
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CRB1, also known as protein crumbs homolog 1, is homologous to Drosophila Crumbs, a protein that is essential for establishing and maintaining apico-basal polarity in epithelia derived from the ectoderm (PMID: 2344615). CRB1 protein is a single transmembrane protein with a large extracellular part, containing three laminin A G domains and 19 epidermal growth-factor-like domains (PMID: 15494026, 18407265, 11734541). The expression of CRB1 is restricted to the retina and brain (PMID: 10508521). Mutations in CRB1 gene cause severe retinal dystrophies, ranging from retinitis pigmentosa (RP) to Leber congenital amaurosis (LCA) (PMID: 11734541).

实验方案

Product Specific Protocols
IHC protocol for CRB1 antibody 26598-1-APDownload protocol
IF protocol for CRB1 antibody 26598-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols