验证数据展示
经过测试的应用
| Positive IHC detected in | mouse eye tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse eye tissue |
| Positive IF/ICC detected in | SH-SY5Y cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26598-1-AP targets CRB1 in IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, IF/ICC, IF-P, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag24512 Product name: Recombinant human CRB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 30-188 aa of BC136271 Sequence: NNTRCLSNSCQNNSTCKDFSKDNDCSCSDTANNLDKDCDNMKDPCFSNPCQGSATCVNTPGERSFLCKCPPGYSGTICETTIGSCGKNSCQHGGICHQDPIYPVCICPAGYAGRFCEIDHDECASSPCQNGAVCQDGIDGYSCFCVPGYQGRHCDLEVD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | crumbs homolog 1 (Drosophila) |
| 别名 | Crumbs homolog 1, crumbs homolog 1 (Drosophila), LCA8, Protein crumbs homolog 1, RP12 |
| 计算分子量 | 1406 aa, 154 kDa |
| 观测分子量 | 150 kDa |
| GenBank蛋白编号 | BC136271 |
| 基因名称 | CRB1 |
| Gene ID (NCBI) | 23418 |
| RRID | AB_2918104 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P82279 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CRB1, also known as protein crumbs homolog 1, is homologous to Drosophila Crumbs, a protein that is essential for establishing and maintaining apico-basal polarity in epithelia derived from the ectoderm (PMID: 2344615). CRB1 protein is a single transmembrane protein with a large extracellular part, containing three laminin A G domains and 19 epidermal growth-factor-like domains (PMID: 15494026, 18407265, 11734541). The expression of CRB1 is restricted to the retina and brain (PMID: 10508521). Mutations in CRB1 gene cause severe retinal dystrophies, ranging from retinitis pigmentosa (RP) to Leber congenital amaurosis (LCA) (PMID: 11734541).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CRB1 antibody 26598-1-AP | Download protocol |
| IHC protocol for CRB1 antibody 26598-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
