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验证数据展示

  • IF Staining of human appendicitis using CL488-68033

    IF Staining of human appendicitis using CL488-68033

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite® Plus 488 CD35 antibody (CL488-68033, Clone: 1B8E6 ) at dilution of 1:200.

  • IF Staining of human appendicitis using CL488-68033

    IF Staining of human appendicitis using CL488-68033

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using CoraLite® Plus 488 CD35 antibody (CL488-68033, Clone: 1B8E6 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated CD35 Monoclonal antibody

CD35 Monoclonal Antibody for IF-P
Cat No. CL488-68033

产品说明书

CloneNo. 1B8E6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF-P

别名

KN, CR1, Complement receptor type 1, C3BR, C3b/C4b receptor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inhuman appendicitis tissue
Planning an IHC experiment? We recommend our IHCeasy CR1/CD35 Ready-To-Use IHC Kit. CR1/CD35 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68033-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68033 targets CD35 in IF-P applications and shows reactivity with human samples.

经测试应用 IF-P Application Description
经测试反应性 human
免疫原 CD35 fusion protein Ag30268 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 complement component (3b/4b) receptor 1 (Knops blood group)
别名 KN, CR1, Complement receptor type 1, C3BR, C3b/C4b receptor
计算分子量 224 kDa
GenBank蛋白编号NM_000573
基因名称 CR1
Gene ID (NCBI) 1378
ENSEMBL Gene IDENSG00000203710
RRIDAB_2934612
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP17927
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CD35, also known as complement receptor type 1 (CR1) or C3b/C4b receptor, is a single-chain glycoprotein consisting of 30 repeating homologous protein domains known as short consensus repeats (~60 amino acids each) followed by transmembrane and cytoplasmic domains (PMID: 8765013). CD35 is variably expressed by granulocytes, monocytes, B cells, some T cells, erythrocytes, follicular dendritic cells, Langerhans cells, and glomerular podocytes (PMID: 19004497). CD35 acts as a receptor for C3b and C4b and plays important roles in the regulation of the complement cascade and clearance of immune complexes. A soluble form of CD35 has been found in plasma (PMID: 3156931). It has been reported that soluble CD35 is produced by a proteolytic cleavage in the C-terminal region of CD35 transmembrane domain (PMID: 9405292).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CD35 antibody CL488-68033Download protocol
Standard Protocols
Click here to view our Standard Protocols