验证数据展示
经过测试的应用
| Positive WB detected in | Raji cells, Ramos cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
33114-1-AP targets CPTP in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag35748 Product name: Recombinant human GLTPD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-214 aa of NM_001029885 Sequence: MDDSETGFNLKVVLVSFKQCLDEKEEVLLDPYIASWKGLVRFLNSLGTIFSFISKDVVSKLRIMERLRGGPQSEHYRSLQAMVAHELSNRLVDLERRSHHPESGCRTVLRLHRALHWLQLFLEGLRTSPEDARTSALCADSYNASLAAYHPWVVRRAVTVAFCTLPTREVFLEAMNVGPPEQAVQMLGEALPFIQRVYNVSQKLYAEHSLLDLP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glycolipid transfer protein domain containing 1 |
| 别名 | Ceramide-1-phosphate transfer protein, GLTP domain-containing protein 1, GLTPD1, Glycolipid transfer protein domain-containing protein 1 |
| 计算分子量 | 24 kDa，214aa |
| 观测分子量 | 24 kDa |
| GenBank蛋白编号 | NM_001029885 |
| 基因名称 | GLTPD1 |
| Gene ID (NCBI) | 80772 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q5TA50 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Ceramide-1-phosphate transfer protein (CPTP) is a member of the glycolipid transfer protein (GLTP) family. It is the only identified protein in mammals that can selectively transport ceramide-1-phosphate (C1P) through a non-vesicular mechanism (PMID: 35982909). CPTP plays a crucial role in various biological processes, including cell proliferation, migration, and invasion. It is involved in the regulation of lipid metabolism, particularly in the context of ceramide-1-phosphate, which is a bioactive sphingolipid with roles in cell signaling, inflammation, and autophagy (PMID: 29164996).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CPTP antibody 33114-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
