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CPSF4 Polyclonal antibody

CPSF4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 15023-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, IF/ICC, IP, ELISA and More (1)

Neb 1, NAR, CPSF30, CPSF 30 kDa subunit, Cleavage and polyadenylation specificity factor subunit 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inJurkat cells, HepG2 cells, HeLa cells, HEK-293 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15023-1-AP targets CPSF4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse
文献引用反应性human, pig
免疫原 CPSF4 fusion protein Ag7103 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cleavage and polyadenylation specific factor 4, 30kDa
别名 Neb 1, NAR, CPSF30, CPSF 30 kDa subunit, Cleavage and polyadenylation specificity factor subunit 4
计算分子量 30 kDa
观测分子量27-30 kDa
GenBank蛋白编号BC003101
基因名称 CPSF4
Gene ID (NCBI) 10898
RRIDAB_2084544
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95639
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The mature 3-prime ends of cellular mRNAs are producted by endonucleolytic cleavage of the primary transcripts, followed by polyadenylation of the upstream cleavage products. The cleavage and polyadenylation specificity factor (CPSF) has a essential role in pre-mRNA 3-prime end processing in that it is required in both the cleavage step and subsequent poly(A) addition [PMID: 9651582]. CPSF4 is a component of the cleavage and polyadenylation specificity factor (CPSF) complex, whose other components are CPSF1, CPSF2, CPSF3 and FIP1L1.

实验方案

Product Specific Protocols
WB protocol for CPSF4 antibody 15023-1-APDownload protocol
IHC protocol for CPSF4 antibody 15023-1-APDownload protocol
IF protocol for CPSF4 antibody 15023-1-APDownload protocol
IP protocol for CPSF4 antibody 15023-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Rep

LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation

Authors - Nasha Zhang
humanWB,IP

Mol Oncol

CPSF4 activates telomerase reverse transcriptase and predicts poor prognosis in human lung adenocarcinomas.

Authors - Wangbing Chen
  • KD Validated
humanWB,IHC,IF

Cancer Lett

Cleavage and polyadenylation specific factor 4 targets NF-κB/cyclooxygenase-2 signaling to promote lung cancer growth and progression.

Authors - Canhui Yi
  • KD Validated
humanWB,IF

Mol Oncol

CREB-binding protein regulates lung cancer growth by targeting MAPK and CPSF4 signaling pathway.

Authors - Zhipeng Tang
pigWB

Int J Biol Macromol

Key pituitary miRNAs mediate the expression of pig GHRHR splice variants by regulating splice factors.

Authors - Zhaohui Teng
humanWB, IF

Biochim Biophys Acta Mol Cell Res

Cleavage and polyadenylation specific factor 4 promotes colon cancer progression by transcriptionally activating hTERT.

Authors - Qian Yang
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