验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, HepG2 cells, HeLa cells, HEK-293 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15023-1-AP targets CPSF4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 |
CatNo: Ag7103 Product name: Recombinant human CPSF4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-244 aa of BC003101 Sequence: MQEIIASVDHIKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEFFLKAACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPLCRHRHTRRVICVNYLVGFCPEGPSCKFMHPRFELPMGTTEQPPLPQQTQPPAKQRTPQVIGVMQSQNSSAGNRGPRPLEQVTCYKCGEKGHYANRCTKGHLAFLSGQ 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cleavage and polyadenylation specific factor 4, 30kDa |
| 别名 | Neb 1, NAR, CPSF30, CPSF 30 kDa subunit, Cleavage and polyadenylation specificity factor subunit 4 |
| 计算分子量 | 30 kDa |
| 观测分子量 | 27-30 kDa |
| GenBank蛋白编号 | BC003101 |
| 基因名称 | CPSF4 |
| Gene ID (NCBI) | 10898 |
| RRID | AB_2084544 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95639 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The mature 3-prime ends of cellular mRNAs are producted by endonucleolytic cleavage of the primary transcripts, followed by polyadenylation of the upstream cleavage products. The cleavage and polyadenylation specificity factor (CPSF) has a essential role in pre-mRNA 3-prime end processing in that it is required in both the cleavage step and subsequent poly(A) addition [PMID: 9651582]. CPSF4 is a component of the cleavage and polyadenylation specificity factor (CPSF) complex, whose other components are CPSF1, CPSF2, CPSF3 and FIP1L1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CPSF4 antibody 15023-1-AP | Download protocol |
| IHC protocol for CPSF4 antibody 15023-1-AP | Download protocol |
| IF protocol for CPSF4 antibody 15023-1-AP | Download protocol |
| IP protocol for CPSF4 antibody 15023-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Rep LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation | ||
Mol Oncol CPSF4 activates telomerase reverse transcriptase and predicts poor prognosis in human lung adenocarcinomas.
| ||
Cancer Lett Cleavage and polyadenylation specific factor 4 targets NF-κB/cyclooxygenase-2 signaling to promote lung cancer growth and progression.
| ||
Mol Oncol CREB-binding protein regulates lung cancer growth by targeting MAPK and CPSF4 signaling pathway. | ||
Int J Biol Macromol Key pituitary miRNAs mediate the expression of pig GHRHR splice variants by regulating splice factors. | ||
Biochim Biophys Acta Mol Cell Res Cleavage and polyadenylation specific factor 4 promotes colon cancer progression by transcriptionally activating hTERT. |
