验证数据展示
经过测试的应用
Positive WB detected in | Jurkat cells, HepG2 cells, HeLa cells, HEK-293 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
15023-1-AP targets CPSF4 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP |
经测试反应性 | human, mouse |
文献引用反应性 | human, pig |
免疫原 | CPSF4 fusion protein Ag7103 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | cleavage and polyadenylation specific factor 4, 30kDa |
别名 | Neb 1, NAR, CPSF30, CPSF 30 kDa subunit, Cleavage and polyadenylation specificity factor subunit 4 |
计算分子量 | 30 kDa |
观测分子量 | 27-30 kDa |
GenBank蛋白编号 | BC003101 |
基因名称 | CPSF4 |
Gene ID (NCBI) | 10898 |
RRID | AB_2084544 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O95639 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The mature 3-prime ends of cellular mRNAs are producted by endonucleolytic cleavage of the primary transcripts, followed by polyadenylation of the upstream cleavage products. The cleavage and polyadenylation specificity factor (CPSF) has a essential role in pre-mRNA 3-prime end processing in that it is required in both the cleavage step and subsequent poly(A) addition [PMID: 9651582]. CPSF4 is a component of the cleavage and polyadenylation specificity factor (CPSF) complex, whose other components are CPSF1, CPSF2, CPSF3 and FIP1L1.
实验方案
Product Specific Protocols | |
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WB protocol for CPSF4 antibody 15023-1-AP | Download protocol |
IHC protocol for CPSF4 antibody 15023-1-AP | Download protocol |
IF protocol for CPSF4 antibody 15023-1-AP | Download protocol |
IP protocol for CPSF4 antibody 15023-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Cell Rep LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation | ||
Mol Oncol CPSF4 activates telomerase reverse transcriptase and predicts poor prognosis in human lung adenocarcinomas.
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Cancer Lett Cleavage and polyadenylation specific factor 4 targets NF-κB/cyclooxygenase-2 signaling to promote lung cancer growth and progression.
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Mol Oncol CREB-binding protein regulates lung cancer growth by targeting MAPK and CPSF4 signaling pathway. | ||
Int J Biol Macromol Key pituitary miRNAs mediate the expression of pig GHRHR splice variants by regulating splice factors. | ||
Biochim Biophys Acta Mol Cell Res Cleavage and polyadenylation specific factor 4 promotes colon cancer progression by transcriptionally activating hTERT. |