验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, HeLa cells, rat liver tissue |
Planning an IHC experiment? We recommend our IHCeasy CPS1 Ready-To-Use IHC Kit. CPS1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
67630-1-Ig targets CPS1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | rabbit |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | carbamoyl-phosphate synthetase 1, mitochondrial |
| 别名 | 2A12A4, Carbamoyl-phosphate synthase [ammonia], mitochondrial, Carbamoyl-phosphate synthetase I, CPSase I, EC:6.3.4.16 |
| 计算分子量 | 165 kDa |
| 观测分子量 | 140-165 kDa |
| GenBank蛋白编号 | BC140943 |
| 基因名称 | CPS1 |
| Gene ID (NCBI) | 1373 |
| RRID | AB_2882831 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P31327 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CPS1 is an enzyme that catalyzes the first committed step of the hepatic urea cycle, which is important in the removal of excess urea from cells. Expressed primarily in the liver and small intestine, CPS1 can be used as a marker for mitochondria in these tissues. In addition, CPS1 expression appears to be lost in adenocarcinomas of the small intestine.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CPS1 antibody 67630-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
