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验证数据展示

  • WB analysis of HeLa using 24513-1-AP

    WB analysis of HeLa using 24513-1-AP

    WB result of CPS1 antibody (24513-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CPS1 transfected HeLa cells.

  • WB analysis of HepG2 using 24513-1-AP

    WB analysis of HepG2 using 24513-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24513-1-AP (CPS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 24513-1-AP

    IHC staining of human liver cancer using 24513-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24513-1-AP (CPS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CPS1 antibody included
  • WB analysis using 24513-1-AP

    WB analysis using 24513-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24513-1-AP (CPS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO验证

CPS1 Polyclonal antibody

CPS1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 24513-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

Carbamoyl-phosphate synthase [ammonia], mitochondrial, Carbamoyl-phosphate synthetase I, CPSase I, EC:6.3.4.16

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, mouse liver tissue, rat liver tissue, L02 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CPS1 Ready-To-Use IHC Kit. CPS1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24513-1-AP targets CPS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag21605

Product name: Recombinant human CPS1 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 1361-1500 aa of BC140943

Sequence: GILIGIQQSFRPRFLGVAEQLHNEGFKLFATEATSDWLNANNVPATPVAWPSQEGQNPSLSSIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIPLLTNFQVTKLFAEAVQKSRKVDSKSLFHYRQYSAGKAA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carbamoyl-phosphate synthetase 1, mitochondrial
别名 Carbamoyl-phosphate synthase [ammonia], mitochondrial, Carbamoyl-phosphate synthetase I, CPSase I, EC:6.3.4.16
计算分子量 1500 aa, 165 kDa
观测分子量140-165 kDa
GenBank蛋白编号BC140943
基因名称 CPS1
Gene ID (NCBI) 1373
RRIDAB_2879583
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31327
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Carbamoyl phosphate synthetase 1 (CPS1) is the first rate-limiting enzyme in the urea cycle, converting ammonium into carbamoyl phosphate, and plays an intricate role in arginine metabolism and pyrimidine metabolism (PMID: 28376202). CPS1 expression is liver specific and suppressed in HCC cells and tumor tissues at the transcriptional level(PMID: 21281797). CPS1 gene comprises 38 exons that encode a polypeptide of 1500 amino acids and has a molecular weight of 165 kDa.

实验方案

Product Specific Protocols
WB protocol for CPS1 antibody 24513-1-APDownload protocol
IHC protocol for CPS1 antibody 24513-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols