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CPS1 Polyclonal antibody

CPS1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 24513-1-AP

Print datasheet

Synonyms

CPS1, CPSase I

验证数据展示

  • WB analysis of HepG2 using 24513-1-AP

    WB analysis of HepG2 using 24513-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24513-1-AP (CPS1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 24513-1-AP

    IHC staining of human liver cancer using 24513-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 24513-1-AP (CPS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 24513-1-AP

    WB analysis using 24513-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24513-1-AP (CPS1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, rat liver tissue, mouse liver tissue, L02 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24513-1-AP targets CPS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen CPS1 fusion protein Ag21605 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carbamoyl-phosphate synthetase 1, mitochondrial
Synonyms CPS1, CPSase I
Calculated Molecular Weight 1500 aa, 165 kDa
Observed Molecular Weight140-165 kDa
GenBank Accession NumberBC140943
Gene Symbol CPS1
Gene ID (NCBI) 1373
RRIDAB_2879583
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP31327
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Carbamoyl phosphate synthetase 1 (CPS1) is the first rate-limiting enzyme in the urea cycle, converting ammonium into carbamoyl phosphate, and plays an intricate role in arginine metabolism and pyrimidine metabolism (PMID: 28376202). CPS1 expression is liver specific and suppressed in HCC cells and tumor tissues at the transcriptional level(PMID: 21281797). CPS1 gene comprises 38 exons that encode a polypeptide of 1500 amino acids and has a molecular weight of 165 kDa.

实验方案

Product Specific Protocols
WB protocol for CPS1 antibody 24513-1-APDownload protocol
IHC protocol for CPS1 antibody 24513-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols