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CPNE3 Polyclonal antibody

CPNE3 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IP, WB,ELISA

Conjugate

Unconjugated

Cat No : 11186-1-AP

Print datasheet

Synonyms

Copine 3, copine III, CPN3, CPNE3, KIAA0636, PRO1071

验证数据展示

  • WB analysis of Y79 using 11186-1-AP

    WB analysis of Y79 using 11186-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 11186-1-AP (CPNE3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human prostate cancer using 11186-1-AP

    IHC staining of human prostate cancer using 11186-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11186-1-AP (CPNE3 antibody) at dilution of 1:50 (under 10x lens).

  • IP experiment of HepG2 using 11186-1-AP

    IP experiment of HepG2 using 11186-1-AP

    IP result of anti-CPNE3 (IP:11186-1-AP, 4ug; Detection:11186-1-AP 1:300) with HepG2 cells lysate 3400ug.

经过测试的应用

Positive WB detected inY79 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11186-1-AP targets CPNE3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, IP, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CPNE3 fusion protein Ag1634 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name copine III
Synonyms Copine 3, copine III, CPN3, CPNE3, KIAA0636, PRO1071
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC036242
Gene Symbol CPNE3
Gene ID (NCBI) 8895
RRIDAB_2084214
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO75131
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Copines are a family of evolutionarily conserved calcium-dependent phospholipid-binding proteins (PMID: 9430674). They contain two Ca(2+)- and phospholipid-binding domains known as C2 domains. Copines are potentially involved in regulating membrane trafficking and in protein-protein interactions.

实验方案

Product Specific Protocols
WB protocol for CPNE3 antibody 11186-1-APDownload protocol
IHC protocol for CPNE3 antibody 11186-1-APDownload protocol
IP protocol for CPNE3 antibody 11186-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

J Cell Physiol

Circulating exosomal CPNE3 as a diagnostic and prognostic biomarker for colorectal cancer.

Authors - Bo Sun
View Article