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CPN2 Polyclonal antibody

CPN2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 27160-1-AP

Print datasheet

Synonyms

ACBP, Carboxypeptidase N subunit 2, CPN2

验证数据展示

  • WB analysis using 27160-1-AP

    WB analysis using 27160-1-AP

    human plasma tissue were subjected to SDS PAGE followed by western blot with 27160-1-AP (CPN2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 27160-1-AP

    IHC staining of human liver using 27160-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27160-1-AP (CPN2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 27160-1-AP

    IHC staining of human liver using 27160-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27160-1-AP (CPN2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman plasma
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27160-1-AP targets CPN2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human
Immunogen CPN2 fusion protein Ag25801 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name carboxypeptidase N, polypeptide 2
Synonyms ACBP, Carboxypeptidase N subunit 2, CPN2
Calculated Molecular Weight 545 aa, 61 kDa
Observed Molecular Weight83-90 kDa
GenBank Accession NumberBC031569
Gene Symbol CPN2
Gene ID (NCBI) 1370
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22792
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Human carboxypeptidase N (CPN), a member of the CPN/E subfamily of "regulatory" metallo-carboxypeptidases, is an extracellular glycoprotein synthesized in the liver and secreted into the blood, where it controls the activity of vasoactive peptide hormones, growth factors and cytokines by specifically removing C-terminal basic residues. Normally, CPN circulates in blood plasma as a hetero-tetramer consisting of two 83 kDa (CPN2) domains each flanked by a 48 to 55 kDa catalytic (CPN1) domain (PMID:17157876). CPN2 also performs a crucial biological function in the invasion and migration of breast cancer and can be used as a biomarker for effective diagnosis and treatment of breast cancer.

实验方案

Product Specific Protocols
WB protocol for CPN2 antibody 27160-1-APDownload protocol
IHC protocol for CPN2 antibody 27160-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols