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验证数据展示

  • WB analysis using 16949-1-AP (same clone as 16949-1-PBS)

    WB analysis using 16949-1-AP (same clone as 16949-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16949-1-AP (CPLX3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16949-1-PBS in a different storage buffer formulation.

  • IF Staining of rat eye using 16949-1-AP (same clone as 16949-1-PBS)

    IF Staining of rat eye using 16949-1-AP (same clone as 16949-1-PBS)

    Immunofluorescent analysis of (-20°C Acetone) fixed paraffin-embedded rat eye tissue using CPLX3 antibody (16949-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16949-1-PBS in a different storage buffer formulation.

  • IF Staining of rat eye using 16949-1-AP (same clone as 16949-1-PBS)

    IF Staining of rat eye using 16949-1-AP (same clone as 16949-1-PBS)

    Immunofluorescent analysis of (-20°C Acetone) fixed paraffin-embedded rat eye tissue using CPLX3 antibody (16949-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16949-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 16949-1-AP (same clone as 16949-1-PBS)

    IP experiment of mouse brain using 16949-1-AP (same clone as 16949-1-PBS)

    IP result of anti-CPLX3 (IP:16949-1-AP, 3ug; Detection:16949-1-AP 1:300) with mouse brain tissue lysate 6000ug. This data was developed using the same antibody clone with 16949-1-PBS in a different storage buffer formulation.

CPLX3 Polyclonal antibody, PBS Only

CPLX3 Polyclonal Antibody for WB, IF-P, IP, Indirect ELISA
Cat No. 16949-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, IP, Indirect ELISA

别名

complexin 3, Complexin III, Complexin-3, CPX III, CPXIII

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

16949-1-PBS targets CPLX3 in WB, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag10571

Product name: Recombinant human CPLX3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-158 aa of BC018026

Sequence: MAFMVKTMVGGQLKNLTGSLGGGEDKGDGDKSAAEAQGMSREEYEEYQKQLVEEKMERDAQFTQRKAERATLRSHFRDKYRLPKNETDESQIQMAGGDVELPRELAKMIEEDTEEEEEKASVLGQLASLPGLNLGSLKDKAQATLGDLKQSAEKCHVM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 complexin 3
别名 complexin 3, Complexin III, Complexin-3, CPX III, CPXIII
计算分子量 158 aa, 18 kDa
观测分子量 20-23 kDa
GenBank蛋白编号BC018026
基因名称 CPLX3
Gene ID (NCBI) 594855
RRIDAB_2084042
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WVH0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CPLX3, also named as Complexin-3, is 158 amino acid protein, which belongs to the complexin/synaphin family. CPLX3 localizes in the membrane and binds to the SNARE core complex containing SNAP25, VAMP2 and STX1A. CPLX3 positively regulates a late step in synaptic vesicle exocytosis.

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