CPLX1 Recombinant antibody

CPLX1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85874-1-RR

产品说明书

CloneNo. 250131A9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

Complexin-1, CPLX 1, CPX I, CPX1, CPXI

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inmouse brain tissue, SH-SY5Y cells, rat brain tissue, mouse cerebellum tissue, rat cerebellum tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85874-1-RR targets CPLX1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CPLX1 fusion protein Ag0387 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 complexin 1
别名 Complexin-1, CPLX 1, CPX I, CPX1, CPXI
计算分子量 15 kDa
观测分子量 15-20 kDa
GenBank蛋白编号BC002471
基因名称 CPLX1
Gene ID (NCBI) 10815
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO14810
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Complexin 1 (CPLX1) is a member of the complexin/synaphin gene family, which are cytosolic proteins that function in synaptic vesicle exocytosis. Complexins are enriched in neurons where they colocalize with syntaxin and SNAP-25. Complexins bind weakly to syntaxin alone and not at all to synaptobrevin and SNAP-25, but strongly to the SNAP receptor-core complex composed of these three molecules. Compexins also compete with alpha-SNAP for binding to the core complex but not with other interacting molecules, including synaptotagmin I, and regulate the sequential interactions of alpha-SNAP and synaptotagmins with the SNAP receptor during exocytosis. CLPX1 binds to the SNAP receptor complex and disrupts it, leading to the release of transmitters. Alterations of complexins may contribute to the molecular substrate for abnormalities of neural connectivity in severe mental disorders.

实验方案

Product Specific Protocols
WB protocol for CPLX1 antibody 85874-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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