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验证数据展示

  • WB analysis using 86818-2-RR (same clone as 86818-2-PBS)

    WB analysis using 86818-2-RR (same clone as 86818-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86818-2-RR (COX6C antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86818-2-PBS in a different storage buffer formulation.

  • WB analysis using 86818-2-RR (same clone as 86818-2-PBS)

    WB analysis using 86818-2-RR (same clone as 86818-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86818-2-RR (COX6C antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86818-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 86818-2-RR (same clone as 86818-2-PBS)

    IF Staining of HepG2 using 86818-2-RR (same clone as 86818-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COX6C antibody (86818-2-RR, Clone: 251865D9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86818-2-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02689-1

    Cytometric bead array sample test of MP02689-1

    The mean COX6C concentration was determined to be 55.7 ng/mL in PC-3 cell extract based on a 1.1 mg/mL extract load, 443.5 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load, 283.8 ng/mL in Jurkat cell extract based on a 1.2 mg/mL extract load.

  • Cytometric bead array standard curve of MP02689-1

    Cytometric bead array standard curve of MP02689-1

    Cytometric bead array standard curve of MP02689-1, COX6C Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86818-2-PBS. Detection antibody: 86818-1-PBS. Standard: Ag1982. Range: 0.469-60 ng/mL.

  • Affinity and Kinetic Characterization of 86818-2-RR

    Affinity and Kinetic Characterization of 86818-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86818-2-RR against Human COX6C were performed. The affinity constant is 1.43 nM.

COX6C Recombinant monoclonal antibody, PBS Only (Capture)

COX6C Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 86818-2-PBS

产品说明书

CloneNo. 251865D9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

COX6, COX6C Recombinant monoclonal antibody, COX6C Recombinant monoclonal antibody, PBS Only (Capture)

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® COX6C Ready-To-Use IHC Kit. COX6C primary antibody included.

产品信息

86818-2-PBS targets COX6C as part of a matched antibody pair:

MP02689-1: 86818-2-PBS capture and 86818-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1982

Product name: Recombinant human COX6C protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-75 aa of BC000187

Sequence: MAPEVLPKPRMRGLLARRLRNHMAVAFVLSLGVAALYKFRVADQRKKAYADFYRNYDVMKDFEEMRKAGIFQSVK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cytochrome c oxidase subunit VIc
别名 COX6, COX6C Recombinant monoclonal antibody, COX6C Recombinant monoclonal antibody, PBS Only (Capture)
计算分子量 75 aa, 9 kDa
观测分子量9 kDa
GenBank蛋白编号BC000187
基因名称 COX6C
Gene ID (NCBI) 1345
RRIDAB_3745141
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP09669
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cytochrome c oxidase (COX) is the terminal oxidase in the respiratory chain in human and other mammalian cells. The enzyme is composed of 13 different subunits. COX6C(Cytochrome c oxidase subunit 6C) is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

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