验证数据展示

WB analysis using 11460-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11460-1-AP (COX6A1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
IHC staining of human pancreas cancer using 11460-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11460-1-AP (COX6A1 Antibody) at dilution of 1:200 (under 40x lens).
Ready-to-use IHC kits available with COX6A1 antibody included
IHC staining of mouse kidney using 11460-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 11460-1-AP (COX6A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with COX6A1 antibody included
IHC staining of human colon cancer using 11460-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11460-1-AP (COX6A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with COX6A1 antibody included
IHC staining of human kidney using 11460-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11460-1-AP (COX6A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with COX6A1 antibody included
IHC staining of mouse stomach using 11460-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 11460-1-AP (COX6A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with COX6A1 antibody included
IHC staining of rat kidney using 11460-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 11460-1-AP (COX6A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with COX6A1 antibody included
IHC staining of rat salivary gland using 11460-1-AP
Immunohistochemical analysis of paraffin-embedded rat salivary gland tissue slide using 11460-1-AP (COX6A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with COX6A1 antibody included
IHC staining of rat stomach using 11460-1-AP
Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 11460-1-AP (COX6A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with COX6A1 antibody included
IHC staining of human colon cancer using 11460-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11460-1-AP (COX6A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with COX6A1 antibody included
IHC staining of human kidney using 11460-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11460-1-AP (COX6A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with COX6A1 antibody included
IHC staining of human kidney using 11460-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 11460-1-AP (COX6A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with COX6A1 antibody included
IF Staining of MCF-7 using 11460-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using COX6A1 antibody (11460-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Featured Product
KD/KO Validated

COX6A1 Polyclonal antibody

COX6A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 11460-1-AP

产品说明书

引用文献(16)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

COX VIa L, COX VIa-L, COX6AL, Cytochrome c oxidase polypeptide VIa-liver, Cytochrome c oxidase subunit VIA-liver

缓冲液配方: PBS, Azide, Glycerol

PBS, Azide, Glycerol

偶联物: Unconjugated

Unconjugated

规格	价格	库存

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概述

经过测试的应用
产品信息
发表文章 (16)
实验方案

产品说明书

MSDS

经过测试的应用

Positive WB detected in	mouse liver tissue, NIH/3T3 cells, rat liver tissue
Positive IHC detected in	human pancreas cancer tissue, mouse kidney tissue, rat stomach tissue, human colon cancer tissue, rat kidney tissue, mouse stomach tissue, human kidney tissue, rat salivary gland tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	MCF-7 cells, SH-SY5Y cells

Planning an IHC experiment? We recommend our IHCeasy® COX6A1 Ready-To-Use IHC Kit. COX6A1 primary antibody included.

应用	推荐稀释比
Western Blot (WB)	WB : 1:2000-1:12000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 2 publications below
WB	See 16 publications below
IF	See 1 publications below

产品信息

11460-1-AP targets COX6A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用	WB, IHC, IF/ICC, ELISA Application Description
文献引用应用	WB, IF
经测试反应性	human, mouse, rat
文献引用反应性	human, mouse, rat, pig
免疫原	CatNo: Ag2006 Product name: Recombinant human COX6A1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-109 aa of BC007723 Sequence: MAVVGVSSVSRLLGRSRPQLGRPMSSGAHGEEGSARMWKTLTFFVALPGVAVSMLNVYLKSHHGEHERPEFIAYPHLRIRTKPFPWGDGNHTLFHNPHVNPLPTGYEDE 种属同源性预测
宿主/亚型	Rabbit / IgG
抗体类别	Polyclonal
产品类型	Antibody
全称	cytochrome c oxidase subunit VIa polypeptide 1
别名	COX VIa L, COX VIa-L, COX6AL, Cytochrome c oxidase polypeptide VIa-liver, Cytochrome c oxidase subunit VIA-liver
计算分子量	85 aa, 9 kDa
观测分子量	9.5 kDa
GenBank蛋白编号	BC007723
基因名称	COX6A1
Gene ID (NCBI)	1337
RRID	AB_2085445
偶联类型	Unconjugated
形式	Liquid
纯化方式	Antigen affinity purification
UNIPROT ID	P12074
储存缓冲液	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

COX6A1(Cytochrome c oxidase subunit 6A1, mitochondrial) is also named as COX6AL and belongs to the cytochrome c oxidase subunit 6A family.This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.

实验方案

Product Specific Protocols
IF protocol for COX6A1 antibody 11460-1-AP	Download protocol
IHC protocol for COX6A1 antibody 11460-1-AP	Download protocol
WB protocol for COX6A1 antibody 11460-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse,human	WB,IF	Autophagy PLK1 (polo like kinase 1)-dependent autophagy facilitates gefitinib-induced hepatotoxicity by degrading COX6A1 (cytochrome c oxidase subunit 6A1). Authors - Peihua Luo KD Validated View Article
mouse	WB	Nat Commun MTH1 protects platelet mitochondria from oxidative damage and regulates platelet function and thrombosis Authors - Yangyang Ding View Article
human	WB	Cell Death Dis The ALDH2/PolG2 axis enhances mitochondrial biogenesis via transcriptional regulation of Nrf2 and promotes chemotherapy resistance in acute myeloid leukaemia. Authors - Xiuying Hu View Article
human	WB	Cell Death Dis O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma. Authors - Weicheng Wu View Article
mouse	WB	Cell Rep Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy. Authors - Nicole M Sayles View Article
human	WB	Mol Cell Proteomics HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV. Authors - Daniella H Hock View Article

验证数据展示

WB analysis using 11460-1-AP

IHC staining of human pancreas cancer using 11460-1-AP

IHC staining of mouse kidney using 11460-1-AP

IHC staining of human colon cancer using 11460-1-AP

IHC staining of human kidney using 11460-1-AP

IHC staining of mouse stomach using 11460-1-AP

IHC staining of rat kidney using 11460-1-AP

IHC staining of rat salivary gland using 11460-1-AP

IHC staining of rat stomach using 11460-1-AP

IHC staining of human colon cancer using 11460-1-AP

IHC staining of human kidney using 11460-1-AP

IHC staining of human kidney using 11460-1-AP

IF Staining of MCF-7 using 11460-1-AP

COX6A1 Polyclonal antibody

COX6A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

PLK1 (polo like kinase 1)-dependent autophagy facilitates gefitinib-induced hepatotoxicity by degrading COX6A1 (cytochrome c oxidase subunit 6A1).

MTH1 protects platelet mitochondria from oxidative damage and regulates platelet function and thrombosis

The ALDH2/PolG2 axis enhances mitochondrial biogenesis via transcriptional regulation of Nrf2 and promotes chemotherapy resistance in acute myeloid leukaemia.

O-GlcNAcylation on Rab3A attenuates its effects on mitochondrial oxidative phosphorylation and metastasis in hepatocellular carcinoma.

Mutant CHCHD10 causes an extensive metabolic rewiring that precedes OXPHOS dysfunction in a murine model of mitochondrial cardiomyopathy.

HIGD2A is Required for Assembly of the COX3 Module of Human Mitochondrial Complex IV.

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