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验证数据展示

  • IF Staining of HeLa using CL488-66547

    IF Staining of HeLa using CL488-66547

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 COX4NB antibody (CL488-66547, Clone: 2B8D3 ) at dilution of 1:200.

  • FC experiment of HepG2 using CL488-66547

    FC experiment of HepG2 using CL488-66547

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human COX4NB (CL488-66547, Clone:2B8D3) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated COX4NB Monoclonal antibody

COX4NB Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66547

产品说明书

CloneNo. 2B8D3

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

EMC8, ER membrane protein complex subunit 8, COX4AL, COX4 neighbor, C16orf4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 66547-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66547 targets COX4NB in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 COX4NB fusion protein Ag13776 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 COX4 neighbor
别名 EMC8, ER membrane protein complex subunit 8, COX4AL, COX4 neighbor, C16orf4
计算分子量 210 aa, 24 kDa
观测分子量 24 kDa
GenBank蛋白编号BC001472
基因名称 COX4NB
Gene ID (NCBI) 10328
RRIDAB_2934477
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO43402
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 COX4NB antibody CL488-66547Download protocol
Standard Protocols
Click here to view our Standard Protocols