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验证数据展示

  • IF Staining of mouse lung using CL594-66351

    IF Staining of mouse lung using CL594-66351

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using CoraLite®594 COX2/ Cyclooxygenase 2/ PTGS2 antibody (CL594-66351, Clone: 3G2B9 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal antibody

COX2/ Cyclooxygenase 2/ PTGS2 Monoclonal Antibody for IF-P
Cat No. CL594-66351

产品说明书

CloneNo. 3G2B9

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

IF-P

别名

COX 2, COX2, COX2/ Cyclooxygenase 2/ PTGS2, Cyclooxygenase 2, GRIPGHS, hCox 2, PGG/HS, PGH synthase 2, PGHS 2, PHS 2, PHS II, Prostaglandin G/H synthase 2, Prostaglandin H2 synthase 2, PTGS2

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF-P detected inmouse lung tissue
Planning an IHC experiment? We recommend our IHCeasy COX2/PTGS2 Ready-To-Use IHC Kit. COX2/PTGS2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66351-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66351 targets COX2/ Cyclooxygenase 2/ PTGS2 in IF-P applications and shows reactivity with human samples.

经测试应用 IF-P Application Description
经测试反应性 human
免疫原

CatNo: Ag24721

Product name: Recombinant human COX2/ Cyclooxygenase 2 protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 20-366 aa of BC013734

Sequence: PCCSHPCQNRGVCMSVGFDQYKCDCTRTGFYGENCSTPEFLTRIKLFLKPTPNTVHYILTHFKGFWNVVNNIPFLRNAIMSYVLTSRSHLIDSPPTYNADYGYKSWEAFSNLSYYTRALPPVPDDCPTPLGVKGKKQLPDSNEIVEKLLLRRKFIPDPQGSNMMFAFFAQHFTHQFFKTDHKRGPAFTNGLGHGVDLNHIYGETLARQRKLRLFKDGKMKYQIIDGEMYPPTVKDTQAEMIYPPQVPEHLRFAVGQEVFGLVPGLMMYATIWLREHNRVCDVLKQEHPEWGDEQLFQTSRLILIGETIKIVIEDYVQHLSGYHFKLKFDPELLFNKQFQYQNRIAAE

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 prostaglandin-endoperoxide synthase 2 (prostaglandin G/H synthase and cyclooxygenase)
别名 COX 2, COX2, COX2/ Cyclooxygenase 2/ PTGS2, Cyclooxygenase 2, GRIPGHS, hCox 2, PGG/HS, PGH synthase 2, PGHS 2, PHS 2, PHS II, Prostaglandin G/H synthase 2, Prostaglandin H2 synthase 2, PTGS2
计算分子量 604 aa, 68 kDa
观测分子量 66-70 kDa
GenBank蛋白编号BC013734
基因名称 COX2/PTGS2
Gene ID (NCBI) 5743
RRIDAB_3673500
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35354
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

COX2 (Prostaglandin G/H synthase 2, PTGS2) mediates the formation of prostaglandins from arachidonate. Its subunit structure is homodimer. The fully N-glycosylated PTGS2 is 72-74 kDa and the aglycosylated is 66 kDa (PMID:19656660). It also expresses a band of 39 kDa after unspecific cleavage (PMID:17509125). The 50 kDa band of fragmented PTGS2 has also previously been detected in AD brains (PMID:14724276).

实验方案

Product Specific Protocols
IF protocol for CL594 COX2/ Cyclooxygenase 2/ PTGS2 antibody CL594-66351Download protocol
Standard Protocols
Click here to view our Standard Protocols
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