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验证数据展示

  • WB analysis using 31091-1-AP (same clone as 31091-1-PBS)

    WB analysis using 31091-1-AP (same clone as 31091-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31091-1-AP (CORO1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31091-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 31091-1-AP (same clone as 31091-1-PBS)

    IHC staining of mouse liver using 31091-1-AP (same clone as 31091-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 31091-1-AP (CORO1B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31091-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 31091-1-AP (same clone as 31091-1-PBS)

    IHC staining of human ovary cancer using 31091-1-AP (same clone as 31091-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31091-1-AP (CORO1B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31091-1-PBS in a different storage buffer formulation.

CORO1B Polyclonal antibody, PBS Only

CORO1B Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 31091-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

Coronin-2, Coronin-1B, CORONIN 2, Coronin 1B

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31091-1-PBS targets CORO1B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag34813

Product name: Recombinant human CORO1B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 393-489 aa of BC006449

Sequence: REAYVPSKQRDLKISRRNVLSDSRPAMAPGSSHLGAPASTTTAADATPSGSLARAGEAGKLEEVMQELRALRALVKEQGDRICRLEEQLGRMENGDA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 coronin, actin binding protein, 1B
别名 Coronin-2, Coronin-1B, CORONIN 2, Coronin 1B
计算分子量 498 aa, 54 kDa
观测分子量68 kDa
GenBank蛋白编号BC006449
基因名称 CORO1B
Gene ID (NCBI) 57175
RRIDAB_3669849
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BR76
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CORO1B (Coronin 1B) is an actin-binding protein that controls actin networks at classical lamellipodia (PMID: 32850828). Coro1B localizes to the plasma membrane, regulates lamellipodia formation, and when phosphorylated, it can no longer bind ARP2/3 and inhibit its actin nucleation abilities (PMID: 22619279).

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